以餐厨垃圾水解酸化液为碳源合成PHA研究

PHA PRODUCTION FROM THE LIQUID OF HYDROLYTIC-ACIDOGENIC FERMENTATION OF FOOD WASTE

研究了餐厨垃圾厌氧发酵产挥发性脂肪酸(VFA)的特性,考察了含固率对餐厨垃圾厌氧发酵产VFA的影响。并分析了以餐厨垃圾水解酸化液为碳源,接种量为3%的情况下,4种不同耐盐菌合成PHA过程对VFA不同成分的利用规律。结果表明:不同含固率的发酵液中VFA总量随发酵时间的进行呈上升趋势,第8天达到最大值,且添加120 g餐厨垃圾的发酵液产出的VFA浓度最高,为7 839.76 mg/L。4种耐盐菌中,Cupriavidus necator合成PHA的能力最强,发酵1 d时PHA含量即可达到110.7 mg/L。所用碳源VFA中具有偶数碳原子的酸含量高于奇数碳原子的酸含量,因此在所得PHA中PHB的含量明显高于PHV。

Volatile fatty acids（ VFA） could be obtained from anaerobic fermentation of food waste. The influence of solid content of anaerobic fermentation system on the production of VFA was investigated. The liquid of hydrolytic-acidogenic fermentation was used as carbon source for PHA production by 4 kinds of salt tolerant bacteria with inoculation amount of 3%.The results showed that maximum value of VFA contents were obtained on the 8 th day of fermentation. When 120 g food waste was added in the fermenter,VFA content reached the maximum of 7839. 76 mg/L. PHA yield of Cupriavidus necator was higher than that of other stains,reached 110. 7 mg/L at 1 day of cultivation. The content of VFA with even carbon atom number was higher than those with odd number,which led to the fact that the content of PHB was higher than PHV in the product.