血管性血友病因子在非小细胞肺癌疗效评价中的作用

Clinical Significance of von Willebrand Factor in Therapeutic Evaluation of non-small cell Lung Cancer

目的探讨非小细胞肺癌(NSCLC)患者血管性血友病因子(v WF:Ag)水平及其启动子-1185 A/G基因多态性的发生率与NSCLC之间的相关性和临床意义,为临床筛查NSCLC和疗效评价提供实验依据。方法随机选取50例NSCLC患者和50例正常人群进行v WF:Ag水平检测并采用PCR和限制性内切酶片段长度多态性方法并结合基因测序检测v WF启动子-1185 A/G基因多态性,观察两组各项指标变化情况。结果 NSCLC患者化疗前v WF:Ag水平显著高于正常人群对照组(114.35±23.33%vs 90.55±3.25%,p<0.05);NSCLC患者化疗前v WF:Ag水平显著高于NSCLC患者化疗后(114.35±23.33%vs 99.81±13.42%,P<0.05);NSCLC患者化疗后v WF:Ag水平高于正常人群对照组(99.81±13.42%vs 90.55±3.25%,p<0.05)。各组人群均存在v WF启动子-1185 A/G基因多态性,NSCLC患者组AA型、AG型和GG型分别占24%、52%和24%,A、G等位基因分布频率各为50%;正常人群对照组AA型、AG型和GG型分别占22%、50%、28%,A、G等位基因分布频率为47%和53%,两组基因型和等位基因频率分布无显著差异(P>0.05)。结论 v WF启动子-1185 A/G基因多态性与NSCLC的发生关联不大,但v WF:Ag水平与NSCLC病情轻重程度及疗效有一定的相关性。

Objective To investigate the clinical significance and relationship between non-small cell lung cancer and von willebrand factor and its polymorphism of promoter-1185 A/G. Methods 50 NSCLC patients were selected randomly as test group,50 healthy peoples were enrolled as control group. Venous blood were collected from both groups and v WF： Ag were determined. Meanwhile,the v WF promoter-1185 A/G polymorphism were studied by PCR and restriction fragment length polymorphism. The changes were observed in two groups. Results The level of v WF： Ag in NSCLC patients before chemotherapy was higher than control group（ 114. 35 ± 23. 33 % vs 90. 55 ± 3. 25 %,p〈0. 05） and that after chemotherapy（ 114. 35 ± 23. 33 % vs99. 81 ± 13. 42 %,p〈0. 05）,the level of v WF： Ag in NSCLC patients after chemotherapy was also higher than control group（ 99. 81 ± 13. 42 % vs 90. 55 ± 3. 25 %,p〈0. 05）. The v WF promoter-1185 A/G polymorphisms were found in two groups. The percentages of AA/AG/GG were 24%/52%/24% in NSCLC group,the frequencies of A and G alleles were 50% individually. The percentages of AA/AG/GG were 22%/50%/28% in control group,the frequencies of A and G alleles were 47% and 53%. There were no significant difference in genotype and alleles frequencies distribution between two groups（ p〉0. 05）. Conclusion The changes of v WF： Ag were close related with the occurrence and development of NSCLC,but its promoter-1185 A/G polymorphisms were not associated with the disease.