曝气量对微生物燃料电池脱氮的影响

Effect of aeration rate on nitrogen removal by microbial fuel cells

实验构建生物阴极双室微生物燃料电池,探究在微氧条件下曝气量对其产电性能和阴极脱氮的影响.以乙酸钠为碳源,氯化铵为氮源.实验在25℃温度下,阴极持续曝气,并控制反应器内为微氧状态,富集培养短程硝化反硝化菌群.实现了在特定曝气量条件下生物阴极短程硝化反硝化脱氮.实验结果表明,在曝气量为1.64 mL·min-1的条件下,短程硝化反硝化脱氮效果最好.亚硝态氮积累率为81.70%,总氮去除率达到69.66%,最大稳定电压达0.47 V左右,库伦效率为43.8%,产电效能较好.针对实际污水处理开展相关实验,MFC阴极短程硝化反硝化总氮去除率可达到81.93%,优于全程硝化反硝化.在短程硝化反硝化的微生物群落中,Betaproteobacteria纲和Thauera菌属在短程硝化反硝化中得到了有效的富集,有利于生物脱氮,并且Nitrosomonas菌是主要的氨氧化菌属.

A bio-cathode double chamber microbial fuel cell was constructed to investigate the influence of aeration rate on its electrical generation performance and cathodic nitrogen removal under micro-aerobic conditions.In the experiment,sodium acetate and ammonium chloride were used as carbon source and nitrogen source,respectively.The reactor was started at 25℃.Continuous aeration was applied to the cathode chamber for the micro-aerobic condition to enrich a short-cut nitrification and denitrification microbial community.The short-cut nitrification and denitrification were realized in the biological cathode at a specific aeration rate.The experimental results showed that at an aeration rate of 1.64 mL·min^-1,short-cut nitrification and denitrification were the best.The accumulation rate of nitrite nitrogen was 81.70%,total nitrogen removal rate reached 69.66%,the maximum stable voltage was around 0.47 V,the coulombic efficiency was 43.8%,and the power generation was better.Related experiments on actual sewage treatment were carried out.The total nitrogen removal rate of short-cut nitrification and denitrification in MFC cathode reached 81.93%,which is better than that of the whole process nitrification and denitrification.In microbial community of short-cut nitrification and denitrification,Betaproteobacteria and Thauera were effectively enriched,which is good for biological nitrogen removal.Besides,Nitrosomonas is the main nitrite-oxidizing bacteria.