甜菜红素的生物活性研究

Study on Biological Activity of Betacyanin

本研究的主要目的是进一步明确甜菜红色素提取纯化及其生物活性。通过本研究表明：1．利用体外抗氧化实验，测定了甜菜红素清除DPPH·自由基、ABTS阳离子自由基、羟基自由基和超氧自由基的能力，抑制脂质过氧化的能力和还原力，初步验证了甜菜红素的生物活性，实验结果表明：甜菜红素具有一定的抗氧化活性。甜菜红素抑制脂质过氧化的能力比较强，具有一定的清除自由基的能力和还原力。2．采用HT-29结肠癌细胞作为实验对象对甜菜红素的生物活性进行研究。发现纯化后的甜菜红素在低浓度（25—2500us／mL）时不具备细胞毒性，高浓度（2500～10000us／mL）时具有细胞毒性。利用细胞抗氧化活性（CAA）实验考察甜菜红素的抗氧化活性，发现甜菜红素在50—6750ug／mL的浓度范围内，能够清除细胞内的过氧自由基和氧自由基，具有一定的抗氧化活性。甜菜红素能够提高HT-29细胞过氧化氢酶和超氧化物歧化酶的水平。细胞中丙二醛（MDA）水平上升，谷胱甘肽（GSH）水平下降，但是它们变化的水平和空白对照组没有显著性差异。利用H2O2对HT-29细胞建立氧化损伤模型，进一步考察甜菜红素对细胞的生物活性。在25—100ug／mL的甜菜红素浓度范围内，甜菜红素对HT一29细胞具有较强的氧化防护和一定的氧化修复作用。

The main purpose of this study is to further clarify the extraction and purification of Betacyanin and its biological activity. This study shows that： 1. Using in vitro antioxidant assays, the ability of beet pigments to scavenge DPPH · free radicals, ABTS cation radicals, hydroxyl radicals, and superoxide radicals, the ability to inhibit lipid peroxidation, and the reducing power The bioactivity of betacyanin was preliminarily verified. The experimental results show that betacyanin has certain antioxidant activity. Betacyanin has a stronger ability to in- hibit lipid peroxidation and has a certain ability to scavenge free radicals and reduce power. 2. The biological ac- tivity of betacyanin was studied using HT - 29 colon cancer cells as experimental subjects. It was found that puri- fied betacyanin has no cytotoxicity at low concentrations （25 to 2 500 ug/mL） and cytotoxicity at high concentra- tions （2 500 to 10,000 ug/mL）. The use of cellular antioxidant activity （CAA） experiments to examine the anti - oxidant activity of betacyanin, found that betacyanin in the concentration range of 50 ~ 6 750 ug/mL, can e- liminate intracellular free radicals and oxygen free radicals, with Certain antioxidant activity. Betacyanin im- proves the levels of catalase and superoxide dismutase in HT -29 cells. Malondialdehyde （MDA） levels in- creased in cells, and glutathione （GSH） levels decreased, but the change of level was not significantly different from the blank control group. The oxidative damage model of HT - 29 cells was established using H2 02, and the biological activity of betacyanin on cells was further investigated. In the concentration range of 25 - 100 ug/mL of betacyanin, it had strong oxidative protection and certain oxidative repair effect on HT -29 cells.