miR171家族保守性分析及其在香蕉叶片不同温度处理下的表达分析

Analysis Conservative of miR171 Family and Its Expression in Banana Leaves under Different Temperature Treatment

[目的]研究三明野生蕉和天宝栽培蕉在不同温度处理后mi R171家族成员在叶片中的表达情况,初步探索香蕉mi R171家族成员的抗寒机制。[方法]采用生物信息学分析和实时荧光定量PCR(q PCR)的方法,对香蕉中mi R171家族成员进行研究。[结果]序列比对结果表明,mi R171前体与成熟体序列间有2个保守性较高的重叠区,推测mi R171成熟体可能来自这2个区域。实时荧光定量PCR结果表明,三明野生蕉的mi R171b的相对表达量在0℃时达到最高,而天宝栽培蕉mi R171b则在4℃处理时表达量达到最高;mi R171c和mi R171c*在野生蕉和天宝蕉中具有相似的表达模式,其表达量都在4℃达到最高。[结论]mi R171家族成员可能具有多重调节功能,其家族成员mi R171b,mi R172c和mi R171c*可能在香蕉冷胁迫中发挥重要作用。

[Objective] To explore the function of mi R171 family members in cold resistance mechanism of Sanming＇banana and Tianbao＇banana,the expression pattern of the mi R171 family of banana was investigated in leaves after different temperature stress treatment. [Method]Bioinformatics analysis and quantitative real-time PCR methods were used to study mi R171 family members in bananas. [Result]Sequence alignment showed that there were two highly conserved regions between the mi R171 precursor sequences and the mature sequences,suggesting that the mi R171 mature sequences may mainly originated from these two regions. Quantitative real-time PCR analysis showed that mi R171 b in Sanming＇banana had the highest relative expression at 0℃,while Tianbao＇banana mi R171 b reached the highest expression at 4℃;mi R171 c and mi R171 c＊ had similar expression patterns in Sanming＇banana and Tianbao＇banana,and their expression level reached the peak at 4℃. [Conclusion] mi R171 family may have multiple regulatory functions,and mi R171 b,mi R171 c,and mi R171 c＊ may play important roles in the cold stress of bananas.