血清MCP-1、IL-6、TNF-α表达在脑胶质瘤患者手术疗效评价中的应用

Application of serum MCP-1, IL-6 and TNF-α expression in the evaluation of surgical outcome of patients with glioma

目的探讨血清单核细胞趋化蛋白-1(MCP-1)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达在脑胶质瘤患者手术疗效评价中的应用价值。方法 104例脑胶质瘤患者根据肿瘤病理分级分为高级别组(51例)和低级别组(53例),另选取30例健康志愿者作为对照组,采集血清标本进行MCP-1、IL-6、TNF-α检测,对比几组患者的检测结果。结果脑胶质瘤Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级患者与对照组患者的MCP-1、TNF-α、IL-6水平比较差异有统计学意义(P<0.05)。脑胶质瘤患者的血清IL-6与病理分级呈正相关(r=0.825,P<0.05);TNF-α、MCP-1均与病理分级呈负相关(r=-0.796、-0.892,P<0.05)。低级别组术后2w的TNF-α、IL-6、MCP-1与对照组比较(P>0.05),高级别组术后2w的TNF-α、IL-6、MCP-1水平与低级别组比较(P<0.05)。MCP-1、TNF-α、IL-6单独诊断高低级别脑胶质瘤的准确率分别为80.75%、75.86%、72.88%,三项联合检测对高低级别脑胶质瘤的诊断准确率为95.78%,显著高于各单项指标的诊断准确率(P<0.05)。结论脑胶质瘤术后病理分级与血清MCP-1、IL-6、TNF-α表达相关,检测这三项血清指标能够为临床早期诊断脑胶质瘤高低级别,为评估手术方法与疗效提供依据。

Objective To explore the application value of serum monocyte chemoattractant protein-1（MCP-1）, Interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） expression in the evaluation of surgical outcome of patients with glioma. Method one hundred and four cases of patients with glioma were divided into high-level group（n=fifty-one） and low-level group（n=fifty-three） according to the pathological grade of the tumor. Selected another thirty cases of healthy volunteers as the control group. Collected the serum samples for detecting MCP-1, IL-6 and TNF-α, and compared the test results of several groups. Results The levels of MCP-1, TNF-α and IL-6 in patients with glioma grade Ⅰ, Ⅱ, Ⅲ, Ⅳ compared with control group had significantly different（P〈0.05）. The serum IL-6 levels in patients with glioma were positively correlated with pathological grade（r=0.825, P〈0.05）. TNF-α and MCP-1 levels were negatively correlated with pathological grade（r=-0.796,-0.892, P〈0.05）. There was no significant difference in TNF-α, IL-6 and MCP-1 postoperative 2 weeks between low-level group and control group（P〉0.05）.The levels of TNF-α, IL-6 and MCP-1 in high-level group were significantly different from those in low-level group（P〈0.05）. The accuracy rates of MCP-1, TNF-α and IL-6 alone were 80.75%, 75.86% and 72.88% respectively in the diagnosis and treatment of gliomas, and the diagnostic accuracy of three combined tests for high and low grade gliomas was 95.78%, which was significantly higher than that of individual indexes（P〈0.05）. Conclusion The pathological grade of brain glioma is significantly correlated with the expression of MCP-1, IL-6 and TNF-α. Three serum markers can be used to diagnose the benign and malignant glaucoma in the early stage and evaluate the mothod and efficacy of the operation in accordance with.