苦玄参提取物中苦玄参苷Ⅰ_A质量控制方法的建立

Establishment of Quality Control Method for PicfeltarraeninⅠ_A in Picria fel-terrae Extracts

[目的]建立苦玄参提取物中苦玄参苷Ⅰ_A的鉴别和含量测定方法。[方法]采用薄层色谱法对苦玄参苷进行定性鉴别;采用高效液相色谱法测定提取物中苦玄参苷Ⅰ_A的含量,色谱条件为:Waters Symmetry C_(18)(4.6×250 mm,5μm)色谱柱,流动相为乙腈—水(35∶65),流速1.0 m L/min,检测波长264nm,柱温35℃,进样量20μL。[结果 ]苦玄参苷Ⅰ_A的薄层色谱鉴别方法专属性强;苦玄参苷Ⅰ_A在20.06~104.10μg/m L的质量浓度范围内线性关系良好(R2=0.9992),平均加样回收率为99.90%,RSD=0.53%。[结论]建立的方法专属性强,定量准确性高,适用于苦玄参提取物的质量控制。

[Objective]To establish an identification and content determination method for picfeltarraeninⅠA in Picria fel-terrae extracts. [Methods]The qualitative identification of picfeltarraenin ⅠA was carried out with thin layer chromatography（TLC）method; high performance liquid chromatography（HPLC） method was used to determine the content of picfeltarraenin ⅠA in Picria fel-terrae extracts, and the chromatography conditions were as followed： the chromatographic column was Waters Symmetry C18（4.6 ×250 mm, 5 μm）, the mobile phase was acetonitrile-water（35 ∶65）, the velocity was 1.0 m L/min, the detecting wavelength was 264 nm, the column temperature was 35 ℃, and the injection volume was 20 μL. [Results]The TLC method showed high specialization for identification of picfeltarraenin ⅠA . A good linear relationship was observed in the mass concentration range of 20.06-104.10 μg/m L（R2=0.9992）;the average recovery rate was 99.90%, and the RSD was 0.53 %.[Conclusion]The established method in this study has good specialization in qualitative analysis and has high accuracy in quantitative analysis and can be used in quality control of picfeltarraeninⅠA in Picria fel-terrae extracts.