孕哺期铅暴露对其仔鼠海马组织中突触蛋白表达的影响

The effects of early life lead exposure on the expression of synaptophysin in the hippocampus of mouse pups

探讨母体受铅暴露对其后代小鼠的海马组织中突触蛋白表达的影响。铅暴露是从母体妊娠初期至断奶为止。将醋酸铅（PbAc）溶于去离子蒸馏水中，再分别浓缩至0．1％。0．5％和1％3个组作为仔鼠饮用溶液。在出生21d后，用石墨炉原子光谱吸收法测定铅指标，用免疫组织化学和Western blot检测海马组织中突触蛋白的表达。结果表明，所有铅暴露组中海马组织和血液中的铅指标均显著高于对照组（P〈0．05）。与对照组相比，突触蛋白的表达显著下降（P〈0．05）。母体铅暴露引起的仔鼠海马组织中突触蛋白过少表达可能是铅神经毒性所致。

The present study was undertaken to investigate the effects of maternal lead exposure on expression of synaptophysin in the hippocampus of mice offspring. Lead exposure initiated from beginning of gestation to weaning. Lead acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0. 1%, 0. 5% and 1%, respectively. On the 21^st postnatal day,the Pb levels were also determined by graphite furnace atomic absorption spectrom- etry. The expression of synaptophysin in hippocampus was examined by immunohistochemistry and Western blot. The lead levels in blood and hippocampus of all lead exposure groups were sig- nificantly higher than that of the control group（P〈0.05）. Compared with the control group,the expression of synaptophysin was decreased（P〈0.05）. The low expression of synaptophysin in the hippocampus of pups may contribute to the neurotoxicity associated with maternal Pb exposure.