摘要
目的:观察名方洗心汤对Aβ(1-42)损伤的海马神经元线粒体的调控作用,探讨名方洗心汤防治阿尔茨海默病(Alzheimer's disease,AD)的可能作用机制。方法:提取Wistar新生鼠海马神经元并培养,分正常对照组,Aβ模型组,低、中、高浓度治疗组;Annexin V/PI法检测细胞凋亡率;JC-1荧光法检测线粒体膜电位;流式细胞仪技术和免疫荧光技术分别检测ROS与Cyt-c的蛋白基因表达。结果:洗心汤含药脑脊液对Aβ(1-42)诱导所致细胞凋亡具有显著保护作用。结论:名方洗心汤能够修复线粒体功能,从而提高Aβ(1-42)诱导细胞的存活率。
Objective: To observe the effect of Xixin Decoction( XXD) on mitochondrial fuction in hippocampal neurons injured by Aβ(1-42) and explore the possible mechanism. Methods: We primarily cultured hippocampal neurons of Wistar rats. The neurons were randomly divided into normal group,Aβ(1-42) model group,and low,moderate,high dose XXD groups. Apoptosis rate was detected by Annexin V/PI double staining flow cytometry. JC-1 fluorescence was used to detect mitochondrial membrane potential.Flow cytometry detected ROS. Immunofluorescence technique was used to detect the expression of Cyt-c protein gene. Results:The cerebrospinal fluid with XXD has a significant protective effect on Aβ(1-42)-induced apoptosis. Conclusion: XXD can repair mitochondrial function and the survival rate of Aβ(1-42) induced cells has been increased.
作者
邵怡然
第五永长
曾健
王芳
翟家明
李婷
SHAO Yiran;DIWU Yongchang;ZENG Jian;WANG Fang;ZHAI Jiaming;LI Ting(First Clinical Medical College of Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China)
出处
《辽宁中医杂志》
CAS
2018年第6期1281-1284,I0004,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81373703
81674042)