Structural insights into the CRISPR-Cas-associated ribonuclease activity of Staphylococcus epidermidis Csm3 and Csm6: a step for the development of novel genome editing tool

CRISPR-Cas systems has been developed as genome editing tools,which are originally discovered as sequence-specific defense system utilized by bacteria and archaea to fight with the invasion by foreign nucleic acids such as phages or plasmids.According to the current classification groups of CRISPR-Cas system,Type I,Type III and Type IV comprise the Class 1 systems for their function through multisubunit Cas protein complex to interference the targeted nucleic acids.In contrast,Class 2 systems,consisting of

CRISPR-Cas systems has been developed as genome editing tools, which are originally discovered as sequence-specific defense system utilized by bacteria and archaea to fight with the invasion by foreign nucleic acids such as phages or plasmids.