芦荟苷对食管癌细胞系KESY70增殖、凋亡和侵袭的影响

Effects of Aloin on Proliferation, Apoptosis and Invasion of Esophageal Cancer KESY70 Cell Line

目的探讨芦荟苷对食管癌细胞系KESY70增殖、凋亡和侵袭的影响。方法用不同浓度的芦荟苷处理食管癌细胞系KESY70。食管癌KESY70细胞随机分为5组:未处理组(KESY70)、对照组(DMSO)和芦荟苷(10、40、80μmol/L)组。CCK-8检测细胞活力和增殖能力。流式细胞术分析细胞凋亡,Transwell检测细胞侵袭能力,蛋白印记法分析PCNA、Cleaved caspase-3和MMP-9的表达。结果与DMSO组相比,40、80和120μmol/L芦荟苷处理组细胞活力明显减弱(P<0.01)。40和80μmol/L的芦荟苷处理3 d后,芦荟苷组细胞增殖能力明显降低(P<0.05),芦荟苷组的细胞凋亡率明显高于DMSO组(P<0.05),侵袭能力受到明显抑制(P<0.05),PCNA、MMP-9蛋白的表达明显下降(P<0.05),Cleaved caspase-3表达明显升高(P<0.05)。结论芦荟苷可抑制食管癌细胞系KESY70的增殖和侵袭,促进细胞凋亡。

Objective To explore the effect of aloin on the proliferation, apoptosis and invasion of esophageal cancer KESY70 cell line. Methods Different concentrations of aloin were added into KESY70 cells which were randomly divided into five groups： untreated group （KESY70）, control group （DMSO） and Aloin groups （10, 40, 80 μmol/L）. CCK-8 assay was used to test the viability and proliferation of KESY70 cells. The apoptosis was detected by flow cytometry. Transwell assay was performed to analyze the invasion of KESY70 cells. The expression levels of PCNA, cleaved caspase-3 and MMP-9 were checked by Western blot. Results Cell viabilities of 40, 80 and 120 μmol/L Aloin groups were remarkably decreased, compared with DMSO group（P〈0.01）. The proliferation rates of KESY70 cells treated with 40 and 80 μmol/L aloin for three days were attenuated, compared with the DMSO group （P〈0.05）. Compared with DMSO group, the apoptosis rates were increased（P〈0.05）, the invasion abilities were inhibited （P〈0.05）, the expression levels of PCNA and MMP-9 were reduced （P〈0.05）, and the expressions of cleaved caspase-3 were increased （P〈0.05） in Aloin groups. Conclusion Aloin may suppress the proliferation, invasion and induce the apoptosis of KESY70 cells.