云南石林和红河地区彝族献血人群HNA-3基因多态性分布比较

Compirision of the distribution of the genetic polymorphism of HNA-3 in the Yi population between Shilin and Honghe of Yunnan prorince,China

目的了解云南地区彝族人群的人类中性粒细胞抗原-3（HNA-3）的分布频率。方法从昆明血液中心2012年8月—2012年11月采集的无关健康献血者标本中,选出云南石林彝族标本83例、红河彝族人标本120例,应用PCR-SBT法对其HNA-3系统做基因测序分型,使用SPSS20.0做统计学处理。结果 HNA-3a/a、3a/b和3b/b基因型频率在石林彝族献血人群中分别为47%（39/83）、40%（33/83）、13%（11/83）,在红河彝族献血人群中分别为51%（61/120）、44%（53/120）、5%（6/120）,其中红河彝族人群HNA-3b/b基因型频率明显低于石林彝族（P0.05）。结论云南不同地区彝族人群HNA-3基因分布具有差异。

Object To study the distribution frequency of human neutrophil antigen-3 （HNA-3） in the Yi population of Yunnan Shilin and Yunnan Honghe.Methods Peripheral blood samples ranging from August 2012 to November 2012 were collected from Kunming Blood Center：, which were previously acquired from unrelated healthy donors in Yunnan area. The genotyping of HNA-3 was performed via PCR-SBT based sequencing on 83 Shilin Yi samples and 120 Honghe Yi samples. SPSS 20.0 software was adopted for HNA-3 allele frequency analysis. Results The frequencies of HNA-3a/a, HNA-3a/b and HNA-3b/b were 47%（39/83） ,40%（33/83） and 13%（11/83） in the Shilin Yi samples and 51%（61/120） ,44%（53/ 120） and 5% （6/120） in the Honghe Yi population.The frequency of the HNA-3b/b genotype in the Honghe Yi samples was significantly lower than thatin the Shilin Yi group （ P〈0. 05 ） .The risk of HNA-3a alloimmunization were 9.63% and 6. 94%, respectively with nostatistically significant difference.Conclusion The distribution of HNA-3 gene showes statistically significant difference 2 the Yi population of different Yunnan area.