一测多评法测定贵州产切面红色土茯苓中3种活性成分的含量

Determination of Three Bioactive Components in Smilacis Glabrae Rhizoma by Quantitative Analysis of Multi-components by Single Marker

目的：建立贵州产切面红色土茯苓中5-O-咖啡酰基莽草酸、黄杞苷及落新妇苷的一测多评分析方法,并验证该方法的可靠性和准确性。方法：以落新妇苷为内参物,通过方法学考察,建立贵州产切面红色土茯苓中5-O-咖啡酰基莽草酸、黄杞苷对落新妇苷的相对校正因子,根据建立的相对校正因子计算22批药材中5-O-咖啡酰基莽草酸、黄杞苷的含量,并比较外标工作曲线法、外标一点法与一测多评法的结果,验证一测多评法的准确性和科学性。结果：建立的5-O-咖啡酰基莽草酸、黄杞苷相对于落新妇苷的相对校正因子重复性和耐用性良好;一测多评法计算的22批贵州产切面红色土茯苓药材样品中5-O-咖啡酰基莽草酸、黄杞苷含量与外标工作曲线法、外标一点法的含量测定结果无明显差异（RSD〈4.0%）;样品含量分析结果表明样品中5-O-咖啡酰基莽草酸、黄杞苷和落新妇苷的含量差异较大,分别为0.106 3%-2.692%,0.031 29%-0.677 1%,0.581 2%-4.897%。结论：建立的一测多评法准确可靠,可用于贵州产切面红色土茯苓的多成分质量评价。

Objective： To establishthequantitative analysis of multi-components by single marker（QAMS） of 5-O-caffeoyl shikimic acid,engeletin and astilbin of Guizhou red-section Smilacis Glabrae Rhizoma,and verify the reliability and accuracy of this method. Method： With astilbin asthestandard substance,the relative correction factors（RCF） of 5-O-caffeoyl shikimic acid and engeletin to astilbin of Guizhou red-section Smilacis Glabrae Rhizoma were established by methodological tests. The established RCF was used to calculate the content of 5-O-caffeoyl shikimic acid and engeletin of 22 batches of medicinal samples,compare the results of the one point external standard method and the external standard working curve method,and verify the accuracy and scientificity of QAMS. Result： The RCF of 5-O-caffeoyl shikimic acid and engeletin to astilbin had a good reproducibility and durability. The three methods used to determine the content of 5-O-caffeoyl shikimic acid and engeletin of 22 batches Guizhou red-section Smilacis Glabrae Rhizoma showed no significant difference（RSD 4. 0%）. The determination results of 5-O-caffeoyl shikimic acid,engeletin and astilbin were different,which were in the range of 0. 106 3%-2. 692%,0. 031 29%-0. 677 1%,0. 581 2%-4. 897%. Conclusion： The QAMS is accurate and reliable,which can be used to evaluate the multi-componentquality of Guizhou redsection Smilacis GlabraeRhizoma.