摘要
目的:观察清燥救肺汤对荷Lweis小鼠肺癌细胞增殖相关糖酵解乳酸生成的影响,探讨其作用机制。方法:50只雄性C57BL/6J小鼠,随机分为模型组,化疗组,清燥救肺汤高、中、低剂量组,每组10只。右腋下注射Lewis肺癌细胞建立肺癌荷瘤模型,清燥救肺汤高、中、低剂量组以11,5.5,2.75 g·kg(-1)·d(-1)造模前2周灌胃给药,化疗组以50 mg·kg(-1)·(2d)(-1)腹腔注射给药,模型组以等体积生理盐水灌胃给药,接种后继续给药2周后处死各组小鼠并取瘤称重,蛋白免疫印迹法(Western blot)检测低氧诱导因子-α亚基(hypoxia inducible factor,HIF-)α),原癌基因蛋白(cancer-myc,C-myc)表达,酶联免疫吸附测定法(ELISA)检测乳酸水平以及乳酸脱氢酶-A(lactate dehydrogenase-A,LDH-A)活性。结果:与模型组比较,清燥救肺汤高、中、低剂量组及化疗组瘤质量,乳酸含量显著降低(P〈0.01);清燥救肺汤高、中、低剂量组及化疗组中肺癌细胞C-myc蛋白表达,LDH-A活性明显降低(P〈0.05,P〈0.01);清燥救肺汤高、中剂量组及化疗组HIF-1α蛋白表达显著降低(P〈0.01)。结论:清燥救肺汤可通过抑制HIF-1α蛋白表达,导致C-myc蛋白表达下调,从而降低LDH-A活性,减少肺癌细胞糖酵解乳酸的生成,从而达到抑制荷Lewis小鼠肺癌细胞增殖的功效。
Objective: To discuss the effect of Qingzao Jiufei Tang on the lung cancer cells proliferation related production of glycolysis lactic acid in Lewis-bearing mice,and investigate its possible mechanism. Method:50 male C57 BL/6 J mice were randomly divided into model group,chemotherapy group,high,medium and low dose Qingzao Jiufei Tang groups,n = 10 in each group. Animal models were induced with the right subaxillary injection of Lewis cells,and Qingzao Jiufei Tang groups started medication two weeks before modeling(11,5. 5,and 2. 75 g · kg(-1)· d(-1)); in chemotherapy group, drug was given by intraperitoneal injection at50 mg·kg(-1)·(2 d)(-1); same volume of saline was given by intragastric administration after two weeks of drug administratio. After 14 d modeling,the mice were killed and tumors were weighed. The protein expression levels of hypoxia inducible factor(HIF)-1α and cancer-myc(C-myc) were detected by Western blot method,and lactate dehydrogenase-A(LDH-A) activity was detected by enzyme linked immunosorbent assay(ELISA). Result: As compared with model group,the tumor weight,lactic acid content were significantly reduced(P 〈 0. 01),and the protein expression of C-myc in lung cancer cells and LDH-A activity were reduced(P 〈 0. 05,P 〈 0. 01) in Qingzao Jiufei Tang high dose group,medium dose group,low dose group and chemotherapy group(P 〈 0. 01);the protein expression of HIF-1α was significantly reduced in Qinzao Jiufei Tang high and medium dose groups and chemotherapy group(P 〈 0. 01). Conclusion: Qingzao Jiufei Tang can inhibit the proliferation of Lewis mice lung cancer cells by inhibiting the expression of HIF-α protein,down-regulating C-myc protein,reducing the activity of LDH-A and reducing the production of lactic acid in lung cancer cells.
作者
陈江涛
徐彬智
余功
胡桥
谢斌
CHEN Jiang-tao;XU Bin-zhi;YU Gong;HU Qiao;XIE Bin(Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Chin)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第15期120-124,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81660729)
江西省教育厅科技项目(GJJ150833)