超高效液相色谱-荧光检测法测定水果中3种萘衍生物

Determination of 3 Naphthalene-derived Compounds in Fruits by Ultra Performance Liquid Chromatography with Fluorescence Detector

建立了超高效液相色谱-荧光检测法同时测定水果中1-萘乙酰胺(NAD)、1-萘乙酸(NAA)和2-萘氧乙酸(2-NOA)3种萘衍生物的方法。样品经2%甲酸-乙腈提取,Qu ECh ERS法净化,用ACQUITY UPLC BEH phenyl色谱柱分离,超高效液相色谱荧光检测器测定。3种化合物质量浓度在0.005~1.0 mg/L范围内,与色谱峰面积呈良好的线性关系,相关系数(r)>0.9999。当样品中添加水平为0.01、0.1和1.0 mg/kg时,回收率为78.3%~95.3%,相对标准偏差(RSD)为2.8%~9.5%,方法检出限(LOD)为0.002~0.003 mg/kg,方法定量限(LOD)为0.006~0.01 mg/kg。该方法简便、灵敏、准确,适合用于水果中NAD、NAA、2-NOA的同时测定。

A method for simultaneous determination of 3 naphthalene-derived compounds（ 1-naphthyl acetamide（ NAD）,1-naphthalene acetic acid（ NAA） and 2-naphthoxy acetic acid（ 2-NOA）） in fruits by ultra performance liquid chromatography with fluorescence detector was developed. The sample was extracted with 2% formic acid-acetonitrile and purified by Qu ECh ERS method.The analytes were separated on ACQUITY UPLC BEH phenyl column and determined by ultra performance liquid chromatography with fluorescence detector.The results showed that there are good linearities between the peak areas and the concentrations in the range of 0.005～1.0 mg/L,with correlation coefficients（ r） more than 0.9999.Recoveries of 4 compouds ranged from 78.3% to 95.3% when the spiked levels of 0.01,0.1 and 1.0 mg/kg,with the relative standard deviations（ RSDs）were 2.8% ～ 9.5%. The limits of detection（ LODs） of the developed method were 0.002 ～ 0.003 mg/kg,and the limits of quantitation（ LOQs） were 0.006～0.01 mg/kg. The method was simple,sensitive and accurate for simultaneous determination of NAD、NAA and 2-NOA in fruits.