微波辅助骨碎补黄酮的提取及抗氧化活性研究

Microwave-assisted Extraction and Antioxidant Activities of Flavonoids from Drynaria Rhizome

采用微波辅助结合响应曲面优化（RSM）法,对骨碎补黄酮提取中的原料质量浓度、微波功率及微波时间等工艺参数进行了优化,为骨碎补黄酮的定量提取提供了理论依据。考察了骨碎补黄酮清除羟基自由基（OH·）和过氧负离子自由基（O_2^（2–·））的能力。结果表明,骨碎补黄酮的最佳提取条件为：原料质量浓度0.03 g/L、微波功率400 W、微波时间10 min,在此条件下,骨碎补黄酮的提取率最高,达5.57%;抗氧化活性实验结果表明,当提取的骨碎补黄酮的质量浓度分别为2.5和3.5 g/L时,骨碎补黄酮对OH·及O_2^（2–·）的清除率分别为10.12%和35.71%。通过响应曲面确定了提取率与影响因素之间的二次多项函数模型。

A microwave-assisted extraction procedure of flavonoids from drynaria rhizome was developed by response surface methodology（RSM） with alcohol as solvent. The effects of mass concentration of raw material, microwave power and microwave time on the extraction yield of flavonoids were investigated. The antioxidant activities of the resultant flavonoids were estimated by the scavenging rate against hydroxyl radical and peroxyl radical. The results showed that the best extraction conditions of flavonoids were obtained as follows： mass concentration 0.03 g/L, microwave power 400 W, and microwave time 10 min. Under these extraction conditions, the maximum extraction yield of flavonoids was achieved（5.57%）. The evaluation of antioxidant activity suggested that the flavonoids exhibited significant antioxidant activities. When the mass concentration of flavonoids was 2.5 g/L, the maximum hydroxy radical scavenging rate was 10.12%, and when the mass concentration of flavonoids reached 3.5 g/L, the maximum scavenging rate for peroxyl radical was 35.71%. The above experimental results showed that the extraction yield was determined by RSM and quadratic multinomial function model between influence factors, which could provide a theoretical basis for the quantitative extraction of flavonoids from drynaria rhizome.