基于线粒体标记的淮南麻黄鸡遗传结构及其遗传多样性分析

The genetic diversity and population structure of Huainan yellow partridge chickens based on mitochondrial DNA marker

通过探究淮南麻黄鸡的遗传多样性和遗传结构,为淮南麻黄鸡的种质资源保护和开发利用提供遗传背景资料。对淮南麻黄鸡线粒体基因组(mitochondrial genome,mt DNA)控制区全序列进行测序,并结合红色原鸡的序列进行生物信息学分析。淮南麻黄鸡mt DNA控制区全长1 231~1 232 bp,共发现26处单核苷酸变异位点,核苷酸多样性(Pi)为0.006 6,单倍型多样性(Hd)为0.917,平均核苷酸差异(K)为7.573。群体共包含14种单倍型,可以分为A、B、C和E 4个分支分别包括3、3、7和1种单倍型。淮南麻黄鸡在线粒体水平上有较高的多态性,并且来自多个不同的母系,有很好的开发潜力。

This study was to investigate the genetic diversity and population structure of Huainan yellow partridge chickens. The complete D-loop region of mitochondrial DNA （mtDNA） of 32 chickens were sequenced and compared with published data of the red jungle fowl. The D-loop region of Huainan yellow partridge chickens was 1 231-1 232 bp in length. The average haplotype diversity, nucleotide diversity and nucleotide differences were 0.917, 0.006 6, and 7.573, respectively. A total of 14 haplotypes were identified, which belonged to four previously pub- lished clades, i.e. Clades A, B, C, and E, and contained 3, 3, 7, and 1 haplotypes, respectively. The results showed a high mitochondrial D-loop diversity and indicated multiple maternal origins of Huainan yellow partridge chickens. The results in this study will be a reference for conservation and utilization of Huainan yellow partridge chickens.