尖镰孢胁迫下黄瓜茎部蛋白质差异表达分析

Differential expression analysis of cucumber stem proteins induced by Fusarium oxysporum

为揭示尖镰孢胁迫下黄瓜茎部蛋白质差异,以黄瓜枯萎病高抗品系‘D0327’和感病品系‘649’为试验材料,分别接种2个毒力不同的尖镰孢C9和S1菌株后提取黄瓜茎部蛋白,运用双向电泳技术研究了尖镰孢侵染后茎部蛋白质组的变化。结果表明,2个尖镰孢菌胁迫下,抗感材料茎部蛋白表达发生了变化。茎部蛋白质双向电泳图谱与对照图谱进行比较分析,成功鉴定出8个差异蛋白点。强毒力菌株C9胁迫下,感病品系中鉴定出3个差异蛋白,分别是烯醇化酶及亚基、肌动蛋白和放氧增强蛋白,抗病品系中差异蛋白为烯醇化酶及亚基、磷酸丙糖异构酶和核苷酸二磷酸激酶。弱毒力菌株S1胁迫下,感病品系中差异蛋白为Csf-2蛋白,抗病品系中差异蛋白为放氧增强蛋白。这些蛋白分别参与能量代谢、光合作用和胁迫反应。

To reveal the difference in proteins of cucumber induced by Fusarium oxysporum,the resistant line‘D0327＇and susceptible‘649＇were inoculated with two different virulent C9 and S1 strains,and differential proteomics in cucumber stems induced by F.oxysporum was analyzed by two-dimensional gel electrophoresis.The results showed that there were different proteins between resistant and susceptible lines.Eight protein spots were identified by mass spectrometry：enolase and subunits,actin,exothermic protein,phosphate isomerase and nucleotide diphosphate kinase were obtained under the stress of C9,and Csf-2 and exothermic protein were obtained under the stress of S1.All of these proteins were involved in energy metabolism,photosynthesis and response to stress.