谷氨酰胺对脓毒症大鼠急性肠黏膜损伤的保护作用

Effect of glutaminde on septic acute intestinal mucosa injury in rats

目的:探讨谷氨酰胺对脓毒症大鼠急性肠黏膜损伤的保护作用。方法:将SD大鼠随机分为3组,即正常组(A组)、模型组(B组)及谷氨酰胺组(C组),模型组及谷氨酰胺组大鼠腹腔注射0.45 mg/m L的脂多糖(LPS)溶液,1 m L/100g,即4.5 mg/kg,10 min内分3次注射完毕进行造模。造模12 h后模型组给予25.2%(80 kcal)百普素溶液灌胃,5次/d,每次4 m L;谷氨酰胺组在上述造模12 h后除灌注百普素溶液外,另灌胃谷氨酰胺溶液3.75g/(kg·d)。模型组随机分为喂养36 h组(B1组)和喂养72 h组(B2组)。比较各组组织病理及肠组织谷氨酰胺水平。结果:(1)模型组肠组织谷氨酰胺浓度、绒毛长度及黏膜层厚度均低于正常组(均P<0.05);模型组肠黏膜Chiu氏评分与正常组比较差异有统计学意义(P<0.05)。(2)谷氨酰胺组肠组织谷氨酰胺浓度较模型组升高(P<0.05);谷氨酰胺组肠黏膜Chiu氏评分、绒毛长度及黏膜层厚度较模型组有不同程度改善(均P<0.05)。结论:脓毒症大鼠肠组织谷氨酰胺浓度下降,存在肠黏膜损伤,给予谷氨酰胺治疗能改善脓毒症大鼠的急性肠黏膜损伤。

Objective： To investigate the effect of glutamine on septic acute intestinal mucosa injury in a rat model.Methods： SD rats were divided into three groups： normal group（ A）,model group（ B）,and glutamine group（ C）. The group B was divided into a 36-h subgroup（ B1） and a 72 h feeding subgroup（ B2）. The concentrations of intestinal glutamine and histopathological changes were measured. Results： Intestinal glutamine levels in subgroups B1 and B2（ 1. 023 ±0. 039 and 1. 651 ± 0. 036,respectively） were lower than in group A（ 2. 924 ± 0. 135,P〈0. 05）. The lengths of the villus and thicknesses of the mucosal layer in subgroups B1 and B2,and group C were significantly different from those in group A.The ultrastructural changes of intestines in group B were obvious,while those in group C were not. Intestinal glutamine levels in subgroups B1 and B2（ 1. 023 ± 0. 039 and 1. 651 ± 0. 036,respectively） were lower than in group C（ 2. 320 ± 0. 157,P〈0. 05）. The damage of mucosa was lessened in group C. Conclusions： Treatment with glutamine can decrease intestinal mucosal injury in septic rats.