无溶剂体系酶促酸解合成富含1,3-二硬脂酸-2-油酸甘油三酯的工艺研究

Synthesis of 1,3-distearoyl-2-oleoylglycerol by enzymatic acidolysis in solvent-free system

优化了无溶剂体系中固定化1,3-特异性脂肪酶催化高油酸葵花籽油与硬脂酸合成富含1,3-二硬脂酸-2-油酸甘油三酯(SOS)的合成工艺。以SOS体系含量为考察指标,考察了酶的种类、底物摩尔比、反应温度、反应时间的影响。确定无溶剂体系下SOS合成的最佳工艺条件为:特异性脂肪酶为NS40086,酶添加量12%(以底物总质量计),底物(高油酸葵花籽油与硬脂酸)摩尔比1∶12,反应时间4 h,反应温度70℃。在最佳工艺条件下反应产物中SOS含量为66.1%,SSO含量为7.1%,SOO含量为13.5%。

Products rich in 1,3 -distearoyl- 2- oleoylglycerol （SOS） was synthesized by enzymatic ac- idolysis of high - oleic sunflower seed oil and stearic acid catalyzed by immobilized 1,3 - regiospecific li- pase in solvent - free system, and the synthesis process was optimized. The effects of lipase type, molar ratio of high - oleic sunflower seed oil to stearic acid, reaction time and reaction temperature on the con- tent of SOS were investigated. The results showed that the optimal conditions of synthesis of SOS in sol- vent -free system were obtained as follows： with immobilized lipase NS40086 as catalyst and its dosage 12% （based on total mass of substrates）, molar ratio of high -oleic sunflower seed oil to stearic acid 1： 12, reaction time 4 h and reaction temperature 70 ℃. Under these conditions, the content of SOS, SSO and SOO in the product were 66. 1% ,7.1% and 13.5 %, respectively.