玉米胚芽粕清蛋白酶解产物的制备及其抗氧化活性和护肝作用

Preparation, Antioxidant Activity, Protecting Liver-of Hydrolysates from Corn Germ Meal

为开发利用玉米胚芽粕资源,研究玉米胚芽粕清蛋白酶解产物的酶解工艺及其抗氧化活性和护肝活性。研究结果表明:采用酸性蛋白酶酶解玉米胚芽粕清蛋白,在酶解温度44.9℃,p H 3.6,加酶量3 093.8 U/g,酶解时间1.5 h条件下,玉米胚芽粕清蛋白水解物对1,1-二苯基-2-三硝基苯肼的清除率达到最优值为95.15%,水解度为43.13%。采用体外2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法、Fe2+螯合法抗氧化模型考察玉米胚芽粕清蛋白水解物抗氧化活性,结果表明其抗氧化活性与质量浓度呈正相关。体内抗氧化实验结果表明:对于酒精诱导的急性酒精性肝损伤模型小鼠,玉米胚芽粕清蛋白水解物能显著提高体内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)的含量,降低氧化产物丙二醛(MDA)的含量。同时,小鼠肝组织病理学切片结果表明,其对急性酒精性肝损伤模型小鼠的肝脏具有一定的保护作用,能够抑制酒精在体内代谢过程中产生的氧化应激。玉米胚芽粕清蛋白酶解物具有潜在的护肝作用。

In order to develop and utilize the resources of corn germ, we studied the enzymatic hydrolysis process and antioxidant activity of CGMAH（Corn, germ, meal, albumin, hydrolysates） of corn germ meal. The results showed that under the condition of the acidic protease hydrolysis of corn germ meal albumin, hydrolysis temperature 44 ℃, p H 6, enzyme dosage 3 093.8 U/g, enzymolysis time 1.5 h, corn germ albumin hydrolysate for DPPH scavenging rate was optimal. By using the method in vitro, such as ABTS and Fe2 ＋chelating, to investigate the antioxidant activity of corn germ protein hydrolysate. The results showed that the antioxidant activity was positively correlated with the concentration.The experimental results show that the in vivo antioxidant on oxidative damage of mice induced by alcohol, corn germ meal albumin hydrolysate could significantly increase the content of SOD in vivo, GSH-Px and GSH, decreased the content of oxidation products of MDA. At the same time, liver tissue pathological results showed that corn germ albumin hydrolysate has a protective effect on liver injury caused by alcohol.