响应面优化提取小球藻蛋白质及其性质研究

Optimization of Protein Extraction from Chlorella vulgaris Using Respone Surface Methodology and Study on Its Property

采用响应曲面法优化小球藻蛋白质提取工艺并对其抗氧化活性进行表征。以小球藻蛋白质的提取率为指标,考察p H、液料比(V/m)、浸提时间、吐温添加量及超声时间对提取率的影响。在单因素试验的基础上,采用三因素三水平的响应面分析法确定小球藻蛋白提取工艺。结果表明小球藻蛋白质最佳提取工艺条件:p H 8.0、浸提时间6 h、吐温添加量0.1%,提取率达46.39%。在最优条件下提取的小球藻蛋白对DPPH自由基、超氧阴离子自由基和ABTS自由基的半数清除质量浓度分别为1.40 mg/m L,1.45 mg/m L和16.6μg/m L,说明小球藻蛋白质具有一定的抗氧化活性。

The optimization of the extraction process of protein from Chlorella vulgaris was achieved by response surface methodology and its antioxidant activity were characterized. Chlorella protein extraction rate was taken as the index to study the effect of p H, ratio of liquid： material（V/m）, extraction time, tween concentration and ultrasonic time on extraction yield. On the basis of single factor experiment, the three factors and three levels of response surface analysis method was taken to determine the Chlorella protein extraction process. The results showed that a p H of 8.0, a extraction time of 6 hours and a tween concentration of 0.1% were optimal for protein extraction from Chlorella and the extraction ratio was 46.39%. The concentration for 50% of maximal effect（EC50） of Chlorella protein extracted under the optimal condition to DPPH free radicals, superoxide anion radicals and ABTS radicals were 1.40 mg/m L, 1.45 mg/m L and 16.6 μg/m L, which showed that protein extraction from Chlorella vulgaris has certain antioxidant activity.