荧光定量PCR检测Kif2a在男性精液中的表达

The expression of Kif2a detected by fluorescence quantitative PCR in male semen

目的:了解男性精液Kif2a基因的表达情况并探讨其对男性不育的影响。方法:收集精液标本70例并对其进行除杂处理,提取精液细胞总RNA,测定其浓度后逆转录为c DNA,荧光定量PCR检测Kif2a mRNA表达量。根据WHO第5版人类精液检验与处理实验室手册中精液各参数(精子存活率、精子活力和精子密度)的参考范围对样本进行筛选分组(正常组和异常组)。结果:精子存活率及精子活力正常组的Kif2a mRNA表达量均是其异常组的7.54倍,差异有统计学意义(P<0.05);精子密度组正常组Kif2a mRNA表达量与异常组的表达量差异无统计学意义(P>0.05)。结论:Kif2a表达低下的精母细胞可能因为精子细胞纺锤体未能形成,造成精子活力与活率低下,对受孕产生影响。

Objective： To explore the expression of Kif2 a in male semen,and its effects on male infertility. Methods： Seventy semen specimens were collected and purified. The total RNA in all specimens was extracted,and transcripted into c DNA. The Kif2 a m RNA was detected using fluorescence quantitative PCR. The samples were divided into abnomal semen group and normal group according to the semen parameters（ including sperm survival,sperm motility and sperm density） according to the fifth edition of ＂ Laboratory manual for human semen testing and treatment＂ of WHO. Results： The Kif2 a m RNA expression level in normal sperm survival and sperm vitality group was 7. 54 times of abnormal group,and the difference of which was statistically significant（ P〈0. 05）. The difference of the Kif2 a m RNA expression level between normal and abnormal sperm density group was not statistically significant（ P〉0. 05）. Conclusions：The low sperm motility and survival rate may be due to the low expression of Kif2 a in spermatogonial cells caused by sperm cells spindle failing to form,which affects the pregnancy.