4种试剂盒提取病理组织切片中裂头蚴DNA效果的比较

Comparison of four kits for extracting DNA from histopathological sections containing plerocercoid

目的筛选一种快速、简单、高效、价格合理的从病理组织切片中提取猬裂头蚴DNA的试剂盒。方法用4种不同的试剂盒提取病理组织切片(含虫白片/HE染色片)中裂头蚴DNA,通过比较所提取DNA的浓度、纯度、提取率、提取时间、提取价格及PCR产物的亮度,评价4种市售商品试剂盒提取DNA的效果。结果 4种试剂盒提取裂头蚴有虫组织白片DNA的结果:试剂盒1提取DNA的浓度、纯度及提取率最高,提取成本也最高且耗时较短;试剂盒2的提取效果与试剂盒1相近,提取成本较低且时间最短;试剂盒3的提取效果介于试剂盒2与试剂盒4之间,成本最低但时间长达26h;试剂盒4提取效果最低,PCR产物条带也最暗,提取成本较低但耗时较长。4种试剂盒提取HE染色片DNA的结果:试剂盒1与试剂盒2提取的效果相近,两者成本较高耗时较短,试剂盒3与试剂盒4的提取效果相近,两者成本低耗时长,试剂盒3与试剂盒4的提取效果均低于试剂盒1和试剂盒2的提取效果。结论 4种商品试剂盒均能满足裂头蚴感染有虫组织的DNA提取,其中试剂盒2更适用于快速提取。

To screen a rapid, simple, effective and affordable kit for extraction of DNA from the histopathological sections, the DNA was parallel extracted from white/ HE stained slices containing plerocercoid with four kits. By comparing the concentration, purity, extraction rate, time, price and brightness of PCR products, the extraction effect of the four kits was evaluated. Extraction results of white slices showed that the concentration, purity and extraction rate of DNA extracted with kit 1 were the highest, the cost was also the highest but the extration time was the shortest; the extraction effect of kit 2 was similar to that of kit 1, the cost was lower but the extraction time was the shortest; the extraction effect of kit 3 was between kit 2 and kit 4, the cost was the lowest but the extraction time was almost 26 hours; the extraction effect of kit 4 was the lowest and the brightness of PCR products was also darkest, the cost was low but the extraction time was long. Extraction results of HE stained slices： kit 1 was similar to kit 2, both cost were high but extraction time was low, kit 3 was similar to kit 4, both cost was low but extraction time were long, the extraction efficiency of kit 3 and kit 4 was lower than that of kit 1 and kit 2. The four kits can meet the DNA extraction of tissues sections containing plerocercoid, in which kit 2 is more fit for fast extraction.