C反应蛋白基因多态性与低高密度脂蛋白胆固醇的关联研究

Association study of C-reactive protein gene polymorphisms with low High-density lipoprotein cholesterol

目的:探讨C反应蛋白(C-reactive protein,CRP)基因多态性与高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)降低的关系。方法:采用整群抽样的方法,在2009年纳入宜兴市官林和徐舍两个镇社区居民4 156例为研究对象,收集一般人群资料并进行体格检查;抽取早晨空腹外周血检测血脂等生化指标。应用Taq Man探针技术对CRP基因4个标签单核苷酸多态性(tag SNPs)进行基因分型。采用多因素Logistic回归分析CRP基因与HDL-C降低的关系。结果:超敏CRP(hs-CRP)水平在rs1205和rs876537位点不同基因型之间均有显著差异(P<0.001)。Rs876537和rs1205与HDL-C降低有显著关联,隐性模型有统计学意义。在hs-CRP升高组(≥1.0 mg/L)中,rs1205和rs876537隐性模型与HDL-C降低有显著关联。在hs-CRP正常组中,rs10737175位点的变异与HDL-C降低有关。在女性人群中,rs10737175、rs1205和rs876537 TT基因型携带者发生HDL-C降低的风险均显著高于CC/CT基因型携带者,女性rs10737175 TT基因型携带者HDL-C的水平显著低于CC+CT基因型携带者。结论:CRP与HDL-C水平存在负相关,CRP基因3个多态位点rs1205、rs1073715和rs876537变异与HDL-C降低显著关联,尤其在女性人群中更值得关注。

Objective： To investigate the association of C-Reactive Protein （CRP）gene polymorphisms with low high-density lipoprotein cholesterol. Methods ： Subjects were recruited by epidemiological cluster sampling in 2009 from Guanlin and Xushe town of Yixing city, and 4156 individuals were confirmed for final analysis. All subjects had completed physical examination and extracted of peripheral blood tests on an empty stomach blood lipids and other biochemical indicators in the morning. TaqMan probe method for four CRP gene SNPs genotyping. Multiple logistic regression was applied to further evaluate relationships between the SNPs and low-HDL-C. Results： The levels of hsCRP were significantly differet among rs1205 and rs876537 genotypes, P 〈 0.001. Rs876537 and rs1205 were significantly associated with decreased HDL-C, and the recessive model was statistically significant. The polymorphisms of rs 1205 and rs876537 were significantly associated with low-HDL-C in the hs-CRP ≥ 1.0 mg/L group. Rs10737175 variation was significantly associated with low HDL-C in the hs-CRP normal group. Female with rs1205, rs10737175, rs876537 TT genotype were more likely to suffer from low-HDL-C. Female with rs 10737175 TT genotype had a lower HDL-C level than CC ＋ TT carriers. Conclusions： CRP polymorphisms were negatively associated with low-HDL-C level. The CRP gene rs 1205, rs1073715 and rs876537 variations were associated with a significant reduction of HDL-C, and the association deserves more attention in the female population especially.