猕猴桃WRKY转录因子家族全基因组鉴定与分析

Genome-wide Identification and Analysis of WRKY Transcription Factor Family in Kiwifruit

本研究旨在鉴定猕猴桃WRKY基因家族成员,为进一步研究Ac WRKY转录因子在猕猴桃生长和发育进程及生物和非生物胁迫中的调控作用提供信息。利用‘红阳’猕猴桃全基因组数据,通过生物信息学在线分析网站与软件利用的方法,对猕猴桃WRKY转录因子家族成员进行鉴定和系统分析。分析表明:从猕猴桃全基因组中共鉴定出89个Ac WRKY基因,分组鉴定和进化分析显示,Ac WRKY蛋白可分为Ⅰ、Ⅱ和Ⅲ个类型。Ⅰ组共有19个成员,其锌指结构是CX_4CX_(22-23)HXH(C_2H_2类型);Ⅱ组共有61个成员,进一步分为Ⅱa、Ⅱb、Ⅱc、Ⅱd和Ⅱe共5个亚组,分别有4、7、27、11和12个成员,其锌指结构为CX_(4-5)CX_(23)HXH(C_2H_2类型);Ⅲ组有9个成员,其锌指结为CX_7CX_(23-24)HXC(C_2HC类型);Ac WRKY蛋白序列比对及保守基序分析表明,Ac WRKY结构域高度保守,但也存在一定的变异。染色体定位表明Ac WRKY基因不均匀分布于除9号染色体外的28条染色体上,有11个WRKY基因无法定位到染色体上,同时发现部分基因存在串联复制现象。同源拟南芥不同组织的基因表达模式分析表明,33个WRKY基因在植物根、叶、花和果四个器官中均有显著性表达,但相对表达水平存在差异。猕猴桃WRKY基因家族具有丰富的生物学功能,与其它已报道的物种WRKY基因相似,广泛参与植物营养和生殖生长以及环境胁迫等多方面的调控。

The purpose of this study was to identify members of the WRKY gene family in kiwifruit,which could provide information for the further study on the regulation of Ac WRKY transcriptional factors in the growth,development and biotic or abiotic stresses in kiwifruit.The whole genomic data of kiwifruit in ＇Hongyang＇ was utilized,and the members of WRKY transcription factor family of kiwifruit were identified and systematically analyzed by bioinformatics online analysis of the website and software.Analysis revealed that totally 89 kiwifruit WRKY genes were found in kiwifruit genome.The group identification and phylogenetic analysis showed that Ac WRKY protein could be classified into GroupⅠ,GroupⅡand Group Ⅲ.There were 19 members in GroupⅠ,whose zinc-finger motif was CX4CX（22-23） HXH（type C2H2）.GroupⅡhad 61 members in total,which could be further divided into 5 subgroups（GroupⅡa：4 members,GroupⅡb：7 members,GroupⅡc：27 members,GroupⅡd：11 members Group Ⅱ e：12 member）,whose zinc-finger motif was CX（4-5）CX（23） HXH（C2H2 type）.Group Ⅲ had 9 members and the zinc-finger motif was CX7CX（23-24） HXC（C2HC type）.The analysis of the WRKY protein sequence alignment and conserved motif analysis displayed that WRKY domain was highly conserved,but there were also some variations.Chromosomal localization indicated that Ac WRKY gene was unevenly distributed on 28 chromosomes except chr9,and 11 WRKY genes were unable to locate on the chromosome.At the same time,some genes had tandem duplication.Expression patterns analysis in different tissues of homologous Arabidopsis thaliana demonstrated that 33 WRKY genes were significantly expressed in four tissues of plant root,leaf,flower and fruit,but the relative expression levels were different.The WRKY gene family of kiwifruit was rich in biological functions and was similar to WRKY gene of other reported species.It was widely involved in the regulation of plant nutrition,reproductive growth and environmental stress.