香烟烟雾提取物对人支气管上皮细胞SIRT6、Caspase-9表达的影响

Effects of cigarette smoke extract on the expression of SIRT6 and Caspase-9 in human bronchial epithelial

目的观察香烟烟雾提取物（CSE）对人支气管上皮细胞（16HBE）中人沉默信息调节因子6（SIRT6）、Caspase-9表达的影响。方法体外培养16HBE，加入不同浓度新鲜CSE诱导细胞，通过荧光定量聚合酶链反应（PCR）方法及Western blotting方法检测SIRT6及Caspase-9的表达量。比较（0％、5％、7．5％、10％）的CSE对16HBE中SIRT6、Caspase-9表达的影响。结果16HBE经过不同浓度（0%、5％、7．5％、10％）的CSE处理后，SIRT6mRNA表达差异有显著统计学意义（P〈0．01）。两两比较，各组间除5％CSE组和7．5％CSE组比较差异无统计学意义外（P〉O．05），其余各组两两比较差异均有显著统计学意义（P〈0．01）。Caspase-9mRNA表达差异有显著统计学意义（P〈O．01）。两两比较，各组间差异均有显著统计学意义（P〈0．01）。16HBE经过不同浓度（0％、5%、7．5％、10％）的CSE处理后。SIRT6蛋白、Caspase-9蛋白表达差异有显著统计学意义（P〈0．01）。两两比较，各组间差异均有显著统计学意义（P〈0．01或〈O．05）。结论CSE可引起16HBESIRT6、Caspase-9表达的改变。

Objective To observe the effect of cigarette smoke extract （CSE） on the expression of human silencing information regulator protein 6 （SIRT6） and Caspase-9 in human bronchial epithelial cells （16HBE）. Methods In vitro culture of 16HBE, we added different concentrations of fresh CSE to induce cells. The expression of SIRT6 and Caspase-9 were detected by fluorescence quantitative polmerase chain reaction （PCR） and Western blotting. The effects of （0 %, 5 %, 7.5 %, 10 % ）CSE on the expression of SIRT6 and Caspase-9 in 16HBE were compared. Results After 16HBE treatment with different concentrations （0 %, 5 %, 7.5 %, 10 % ） of CSE, the SIRT6 mRNA expression showed significant difference （ P d0.01）. The differences between each two groups were statistically significant except for 5% CSE vs 7.5 % CSE （ P 〉0.05）. Caspase-9 mRNA expression between groups showed significant difference （ P〈0.01）.The differences between each two groups were statistically significant （ P〈0.01）. After 16HBE treatment with different concentrations （0 %, 5 %, 7.5 %, 10 % ） of CSE, the SIRT6 protein expression and Caspase-9 protein showed significant difference between groups （ P〈0.01）. The differences between each two groups were statistically significant （ P〈0.01 or〈0.05）. Conclusions CSE can cause SIRT6, Caspase-9 of 16H13E expression changes.