探讨《驴成分检测实时荧光PCR法》标准中检出限的合理性

Discussion on the rationality of limit of detection in standard of Detection of donkey ingredient-real-time PCR method

目的探讨SN/T3730.4-2013《食品及饲料中常见畜类品种的鉴定方法第四部分:驴成分检测实时荧光PCR法》标准中检出限规定的合理性。方法参考SN/T 3730.4-2013提供的引物及探针序列,采用实时荧光PCR检测方法鉴别市场抽检驴肉的真伪性。结果 Ct值在25~35之间的样品按照SN/T 3730.4-2013标准规定应判为检出驴成分,但通过对上述样品用马源性引物进行扩增和测序得知样品为马肉而非驴肉。结论建议标准应根据不同的检验对象制定不同的检出限,从而降低出现假阳性的概率。

Objective To discuss the reasonability of the limit of detection in standard of SN/T3730.4-2013 Identification of domestic animal ingredient in food and feed-Part 4：Detection of donkey-derived ingredient-real-time PCR method.Methods According to the primers and probe sequences provided by SN/T 37344-2013,the authenticity of sampling donkey meat in the market was identified by real-time fluorescence PCR detection method.Results The samples with Ct value between 25 and 35 should be judged as the donkey component detected in accordance with SN/T 37344-2013 standard.By amplifying and sequencing the above samples with equine primers,it was found that the samples were horse meat instead of donkey meat.Conclusion It is suggested that different limits of detection should be set according to different inspection objects,so as to reduce the probability of false positive.