辣椒粉中非法添加苏丹红色素快速检验分析

Rapid test and analysis of Sudan red illegally added into Capsicum annuum L

目的建立辣椒粉中苏丹红色素快速检验分析方法。方法以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照,以正己烷-乙酸乙酯(7:1,V:V)为展开剂,采用薄层色谱法迚行定性鉴别;以苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ为对照,Agilent C_(18)(250 mm×4.6 mm,5μm)为色谱柱,乙睛-0.2磷酸(92:8,V:V)为流动相,流速为1.0 m L/min,检测波长为495 nm,柱温30℃,采用高效液相色谱法迚行检测。结果建立薄层色谱法能很好分离各成分;苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ的线性范围分别在16.49~245.59μg(r=0.9999)、16.30~334.88μg(r=0.9997)、16.81~478.02μg(r=0.9999)、16.82~438.21μg(r=0.9996);平均回收率(n=6)分别为97.68%、98.35%、98.32%、98.14%。结论本法简便可行、重复性好,可用于辣椒粉中非法添加苏丹红色素的质量控制和快速检验。

Objective To establish a method for rapid detection and analysis of Sudan red in Capsicum annuum L.Methods Sudan red Ⅰ,Sudan red Ⅱ,Sudan red Ⅲ and Sudan red Ⅳ were used as control,hexane-ethyl acetate（7：1,V：V） was used as developing agent,and thin layer chromatography（TLC） was used for qualitative identification.Sudan red Ⅰ,Sudan red Ⅱ,Sudan red Ⅲ and Sudan red Ⅳ were used for control,the chromatographic column was Agilent C_（18）（250 mm× 4.6 mm,5 m）,mobile phase was acetonitrile-0.2 phosphate（92：8,V：V）,flow rate was 1.0 m L/min,detection wavelength was 495 nm,and column temperature was 30 ℃.Samples were detected by high performance liquid chromatography（HPLC）.Results The established TLC method had a good separation effect on each component.The linear ranges of Sudan red Ⅰ,Sudan red Ⅱ,Sudan red Ⅲ and Sudan red Ⅳ were 16.49-245.59 μ g（r=0.9999）,16.30-334.88 μ g（r=0.9997）,16.81-478.02 μ g（r=0.9999） and 16.82-438.21 μ g（r=0.9996）,respectively.The average recovery rates（n=6） were 97.68%,98.35%,98.32% and 98.14%,respectively.Conclusion This method is simple,feasible and reproducible,which can be used for quality control and rapid test of Sudan red pigments added illegally in Capsicum annuum L.