屠宰季节对蒙古羊肌内结缔组织含量及热变性温度影响研究

Study on the effect of season of slaughtering on intramuscular connective tissue content and thermal denaturation temperature of mongolia sheep

以不同屠宰月份自然放养的蒙古羊的半腱肌和肱三头肌结缔组织为研究对象,利用差示扫描量热仪DSC(Different Scanning Calorimeter)测定肌内结缔组织中胶原蛋白的热变性温度,结果如下:相同屠宰月份半腱肌中结缔组织含量高于肱三头肌;半腱肌和肱三头肌中结缔组织含量除5月和8月间变化不显著外其余均显著(p<0.05)。8月结缔组织含量最低,11月最高。肱三头肌结缔组织的热变性温度高于半腱肌;11月屠宰样品中半腱肌结缔组织的热变性温度Td最高。而肱三头肌结缔组织的热变性温度Td在5月屠宰样品中最高。解释了自然放养蒙古羊不同部位骨骼肌内结缔组织特性在不同月份的变化规律,在此基础上为屠宰月份变化,而引起结缔组织的特性变化机理提供理论依据。

The semitendinosus and triceps brachii connective tissue of mongolia sheep which was naturally stocked and slaughtered in different months was taken as research object, and different scanning calorimetry DSC was used to determine denaturation temperature of collagen in intramuscular connective tissue. The results showed that connective tissue content of semitendinosus was higher than that of triceps brachii in the same month of slaughtering; The change of connective tissue content in semitendinosus and triceps brachii was significant except in May and August （p 〈 0.05）. The connective tissue content in August was the lowest, and November was the highest. The thermal denaturation temperature of triceps brachii connective tissue was higher than that of semitendinosus; The thermal denaturation temperature Td of semitendinosus connective tissue in the slaughtered sample of November was the highest, and the thermal denaturation temperature Td of triceps brachii connective tissue in the slaughtered sample of May was the highest. The change law of characteristics of connective tissue in skeletal muscle which was from different sites of mongolia sheep naturally stocked in different months was explained, on this basis, it provided theoretical basis for characteristics change mechanism of connective tissue which was caused by the change of month of slaughtering.