微小核糖核酸-372在原发性肺癌组织中的表达水平及临床意义

Expression level and clinical significance of microRNA-372 in primary lung cancer tissues

目的探讨原发性肺癌组织中微小核糖核酸-372(miRNA-372)的表达水平及临床意义。方法选取肺癌患者75例,采用实时荧光定量PCR法检测患者癌组织及癌旁正常组织中miRNA-372的表达水平,比较不同临床特征患者的肺癌组织miRNA-372表达水平。结果肺癌组织中的miRNA-372相对表达量低于癌旁正常组织(P<0.05);肿瘤直径≥2 cm者、Ⅲ~Ⅳ期者癌组织中miRNA-372相对表达水平分别低于肿瘤直径<2 cm者、Ⅰ~Ⅱ期者(均P<0.05)。结论 miRNA-372在原发性肺癌组织中呈低表达,其可能与肺癌发生发展相关。

Objective To investigate the expression level and clinical significance of microRNA-372（ miRNA-372） in primary lung cancer tissues. Methods Seventy-five patients with lung cancer were enrolled,and the expression levels of miRNA-372 in cancer tissues and adjacent normal tissues were detected by real-time fluorescent quantitative PCR. The expression levels of cancer tissues miRNA-372 were compared among the patients with different clinical features. Results The relative expression level of miRNA-372 in lung cancer tissues was lower than that in adjacent normal tissues（ P〈0. 05）. The relative expression levels of cancer tissues miRNA-372 in the patients with tumor diameter ≥2 cm or of stage Ⅲ-Ⅳ were lower than those in the patients with tumor diameter 2 cm or of stageⅠ-Ⅱrespectively（ all P〈0. 05）.Conclusion The expression of microRNA-372 is quite low in primary lung cancer tissues,which might be related to the occurrence and progression of lung cancer.