头针丛刺疗法对老年痴呆大鼠学习记忆能力及海马乙酰胆碱水平的影响

Effects of cluster needling at scalp point on learning and memory abilities and hippocampal acetylcholine level in rats with Alzheimer's disease

目的观察头针丛刺针疗法对老年痴呆大鼠学习记忆能力及海马组织乙酰胆碱(ACh)水平的影响。方法将32只大鼠随机分为正常组、假手术组、模型组、头针丛刺组各8只。模型组、头针丛刺组进行穹窿—海马伞损害以建立老年痴呆大鼠模型,假手术组打开颅骨但不给予穹窿—海马伞损害,正常组不给予处理。手术后第7天头针丛刺组给予头针丛刺治疗28 d,其他3组进行等时固定。应用Morris水迷宫实验检测大鼠学习记忆能力,酶联免疫吸附法检测海马组织ACh水平。结果实验结束前3 d,模型组大鼠每天达到所经过的路程及逃避潜伏期的时间均长于其余3组,跨越平台次数少于其余3组(均P<0.05);干预结束后,模型组海马ACh水平低于其余3组(P<0.05)。结论头针丛刺法治疗可以改善老年痴呆大鼠学习记忆能力,其机制可能与上调海马组织ACh水平有关。

Objective To observe the effects of cluster needling at scalp point on learning and memory abilities and hippocampal acetylcholine（ ACh） level in rats with Alzheimer＇ s disease（ AD）. Methods Thirty-two rats were randomly divided into normal group,sham group,model group and cluster needling group. The fimbria-fornix injury was developed in the rats of the model group and cluster needling group to establish the rat model of AD,skull opening was conducted in the sham group without inducing fimbria-fornix injury,and no intervention was performed in the normal group. On the 7 th day after operation,the cluster needling group received cluster needling at scalp point,and the treatment lasted for 28 days,meanwhile,the rats of other three groups received fixation with the same duration. Morris water maze test was used to detect the learning and memory abilities of rats,and enzyme-linked immunosorbent assay was used to detect the ACh level. Results Three days before the end of experiment,the daily distance and the duration of avoidance latency of the rats in the model group were longer than those in the other three groups,the number of flatform crossing in the model group was less than that in the other three groups（ all P〈0. 05）; after intervention,the level of hippocampal ACh in the model group was lower than that in the other three groups（ P〈0. 05）. Conclusion Cluster needling at scalp point can improve the learning and memory abilities of AD rats,which might be related to up-regulating the level of hippocampal ACh.