杜鹃花叶片转录组测序数据组装及功能注释

De novo assembly and functional annotation of transcriptome data of Rhododendron pulchurum cv. Baifeng 4 leaf

采用2代Illumina Hi-Seq测序技术对2年生杜鹃花白凤4号(Rhododendron pulchurum cv.Bai Feng 4)扦插苗叶片的转录组进行测序,获得了213 723 424条Clean reads数据,经过质控和De novo。组装共获得平均长度为930 nt的53 568个Unigenes。与Nr、Swiss-Prot、KOG、KEGG四大数据库进行BLAST信息比对(Evalue≤10^(-5)),共获得28 877个注释基因。与Nr数据库序列同源性比较发现,杜鹃花与葡萄(Vitis vinifera)具有较高的同源性,而与其他物种的同源性较低。杜鹃花转录组的Unigenes在KOG数据库比对上30 508个注释,分为25类。在GO数据库比对上17 218个Unigenes,其中与抗逆有关的Unigenes有11 928个。在KEGG数据库的132条代谢通路中富集了6 475个杜鹃花Unigenes,其中注释到植物激素生物合成代谢途径和信号转导途径的有384个。同时,有1 062个Unigenes被注释为转录因子,有8 738条SSR标记被挖掘。

In this study, the transcriptome analysis of two-years-old cuttage potted seedlings of Rhododendron pulchurum cv. Baifeng 4 were carried out by the second generation of Illumina sequencing technology. The results showed that 213 723 424 Clean reads and 53 568 Unigenes whose average length was 930 nt were obtained after filter, quality control and De novo assembly. Based on it, 28 877 Unigenes were successfully annotated to some databases, such as Nr, Swiss-Prot, KOG （clusters of orthologous group）, KEGG （Kyoto encyclopedia of genes and genomes） （E-value≤10 -5 ）. Comparing with the sequence homology of Nr database, it was found that Rhododendron pulchurum had higher homology with Vitis vinifera , but lower homology with other species. There were 30 508 Unigenes from KOG database and divided into 25 categories. GO （gene ontology） analysis showed that 11 928 Unigenes were identified as potentially involved in environmental stresses. From KEGG analysis, 6 478 Unigenes were assigned to 132 known pathways. Among these pathways, 384 Unigenes were related to biosynthesis and transduction of phytohormone. In addition, there were 1 062 Unigenes were annotated as transcription factors, a total of 8 738 SSR markers were excavated, which laid the foundation for further study of Rhododendron .