苹果酸酶3(ME3)对人脑胶质瘤细胞的增殖、迁移、侵袭及间质转换的影响

Effects of ME3 on the Abilities of Proliferation, Migration, Invasion and Mesenchymal Transition in Human Glioma Cells

该研究探讨了苹果酸酶3(malic enzyme 3,ME3)对人脑胶质瘤细胞增殖、迁移、侵袭和间质转换能力的影响。首先,用Real-time PCR和Western blot检测胶质瘤细胞中ME3的m RNA及其蛋白质的水平。使用质粒(sh-ME3)转染高表达ME3的脑胶质瘤细胞U87MG和U251MG,CCK-8和克隆形成实验分别检测细胞增殖以及克隆形成能力。Transwell实验检测细胞迁移和侵袭能力,并且采用细胞划痕实验进一步检测细胞的迁移能力。用Western blot检测干扰ME3后细胞间质表型标志物。结果表明,ME3下调后,U87MG和U251MG细胞的增殖、迁移和侵袭能力减弱,并且胶质瘤间质表型标志物的表达明显降低,间质转换能力被抑制。以上结果说明,ME3在脑胶质瘤细胞的增殖、迁移和侵袭中发挥了重要作用,同时,ME3因其在胶质瘤发展中发挥的重要作用可能成为肿瘤治疗的新靶点。

The purpose of this study is to investigate the effects of malic enzyme 3（ME3） on the abilities of proliferation, migration, invasion and mesenchymal transition in human glioma cells. We first detected the relative levels of m RNA and protein of ME3 in different glioma cell lines, respectively. Then the specific plasmids（sh-ME3） were transfected into the glioma cells U87 MG and U251 MG. The effects of ME3 on cell proliferation and colony formation were detected by CCK-8 assay and clone formation assay, respectively. Transwell assays were used to examine the ability of ME3 on cell migration and invasion, and wound healing assay was also used to measure the cell migration. Western blot was used to examine the effect on the mesenchymal transition of glioma cells with the ME3 knockdown. The results showed that the ME3 knockdown decreased the ability of proliferation, migration and invasion in U87 MG and U251 MG cells, and also inhibited the expression of mesenchymal markers and mesenchymal transition of the glioma cells. Collectively, the present study demonstrates that ME3 plays a critical role in the development of glioma and may provide a potential therapeutic target for it.