中药牛大力对尿酸盐诱导小鼠模型滑膜细胞炎症的影响

Influence of Chinese herbal medicine Millettia speciosa champ on the synovial cells inflammation of mouse model induced by urate

目的探讨中药牛大力对尿酸盐诱导小鼠模型滑膜细胞炎症的影响。方法 SPF级雄性Wistar小鼠60只随机分成正常对照组、模型组和研究组[牛大力低(4.55 g/kg)、中(9.10 g/kg)、高(13.65 g/kg)剂量组],每组各10只。除正常对照组外连续给药7 d,正常对照组及模型组不做任何给药,每日灌胃等量的生理盐水,其余四组均通过小鼠右侧踝关节注射尿酸盐制造急性痛风性关节炎模型,检测比较造模前和注射尿酸盐48 h的血清一氧化氮(NO)、白细胞介素1β(IL-1β)、肿瘤坏死因子(TNF-α)、前列腺素E2(PGE2)等炎症因子水平和血清尿酸(UA)水平。模型组验膜成功后脱颈处死并取关节囊和滑膜组织行HE染色观察小鼠右侧踝关节滑膜组织病理改变情况,牛大力各剂量组以牛大力低、中、高剂量煎制成汤药,每天上午9点灌胃治疗1次,均连续治疗7 d。治疗3、7 d时再次检测比较牛大力低、中、高剂量组的血清炎症因子水平和血清UA水平,并在治疗7 d后两组滑膜组织行HE染色观察小鼠右侧踝关节滑膜组织病理改变情况。结果四组注射尿酸盐48 h的血清NO水平较建模前降低,而血清IL-1β、TNF-α、PGE2等炎症因子水平和血清UA水平均高于建模前,差异有统计学意义(P<0.05)。HE染色观察显示,牛大力中、高两剂量组滑膜组织血管充血肿胀,炎性细胞浸润明显。牛大力低、中、高剂量组治疗3、7 d的血清NO水平均明显高于模型组,而血清炎症因子水平和血清UA水平均低于模型组,差异有统计学意义(P<0.05);牛大力低、中、高剂量组治疗3、7 d的血清NO水平均明显高于治疗前,而血清炎症因子水平和血清UA水平均低于治疗前,差异有统计学意义(P<0.05)。治疗7 d后HE染色观察显示,牛大力低、中、高剂量滑膜组织炎症浸润改善,病症缓解,但模型组小鼠滑膜组织炎症无明显改善。结论中药牛大力有助于缓解尿酸盐诱导小鼠模型滑膜细胞炎症,值得推广。

Objective To investigate the influence of Chinese herbal medicine Millettia speciosa champ on the synovial cells inflammation of mouse model induced by urate. Methods A total of 60 male Wistar mice of grade SPF were randomly divided into the normal control group, the model group, and the study group（low doses group of 4.55 g/kg, middle does group of 9.10 g/kg, and high doses group of 13.65 g/kg）, 10 cases in each group. Except for the normal control group, the drug was continuously given to 7 d, while the normal control group and the model group did not take any medicine, and given the same amount of normal saline daily to the stomach, the other four groups were injected urate into the right ankle joint to create an acute gouty arthritis model. The levels of serum nitric oxide（NO）, interleukin-1β（IL-1β）, tumor necrosis factor（TNF-α）, prostaglandin E2（PGE2）, and serum uric acid（UA） were measured and compared between before model establishment and after injection of urate 48 h. After successful examination, the model group was sacrificed and the joint capsule and synovial tissue were selected. HE staining was used to observe the pathological changes of the synovial tissue of the right ankle joint in mice. Each dose group was decocted with low,medium and high doses of Millettia speciosa champ, each group was treated with gavage for 1 times at 9 am, for 7 consecutive days. In the treatment of 3 and 7 d, the levels of serum inflammatory factors and serum UA levels in the low,medium and high doses groups of Millettia speciosa champ were measured again, and after 7 d treatment, the synovial tissues of two groups were stained with HE to observe the pathological changes of synovial tissue in the right ankle. Results The level of serum NO in four groups after injection of urate 48 h was lower than that before modeling, while the serum levels of IL-1β, TNF-α, PGE2 and serum UA were higher than those before the modeling, with significant difference（P 0.05）. HE staining showed that there was congestion and swelling in the blood vessels of synovial tissue in the medium and high doses groups of Millettia speciosa champ, and the infiltration of inflammatory cells was obvious. The serum NO levels in the low, medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were significantly higher than those in the model group, and the level of serum inflammatory factors and serum UA in the low, medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were lower than that in the model group, with significant difference（P〈0.05）. The serum NO levels in the low, medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were significantly higher than those before treatment, and the level of serum inflammatory factors and serum UA in the low, medium and high dose groups of Millettia speciosa champ in the treatment of 3 and 7 d were lower than that before treatment, with significant difference（P〈0.05）. After treatment for 7 d, HE staining showed that the inflammatory infiltration of the synovial tissue in the low, middle and high doses group of Millettia speciosa champ was improved and the disease was relieved, but there was no obvious improvement in the synovial tissue inflammation in the model group. Conclusion Chinese herbal medicine Millettia speciosa champ can help alleviate the inflammation of the mouse model synoviocytes induced by urate, which is worthy of popularizing.