拟南芥AT4G32010基因缺失突变体对非生物胁迫的响应

Response of Arabidopsis AT4G32010 Gene Deleted Mutant to Abiotic Stress

B3家族基因是植物中特有的转录因子,参与到植物生长发育、抗逆境胁迫等过程。拟南芥HSI基因(AT4G32010基因)是B3家族中成员之一。以拟南芥野生型和突变体hsi为材料,利用PCR和实时荧光定量PCR技术获得HSI基因表达量发生变化、纯合突变体。在模拟干旱和盐胁迫下,野生型和突变体his发芽率随甘露醇和盐浓度的增加而下降。同一浓度下,突变体hsi发芽率均低于野生型,两者之间差异明显。表明拟南芥HSI基因在种子发芽过程中起正调控作用。在模拟干旱和不同盐浓度胁迫下,野生型和突变体根长度差异不明显。表明该基因参与根的生长过程,关于其调控作用还需要进一步分析。

B3 family genes are unique transcription factors in plants and participate in the process of plant stress resistance.Arabidopsis thaliana HSI gene（AT4G32010） is a member of B3 family. In this study, homozygous mutant hsi were obtained by using PCR. The expression of HSI gene in mutant is lower than that in wild type. Under drought and salt stress, the germination rate of wild type and mutant hsi decreased with the increase of mannitol and salt concentration. At the same concentration, the germination rate of the mutant hsi was lower than that of the wild type, and the difference was significant.The results indicated that HSI gene of Arabidopsis thaliana played a positive role in seed germination. Under drought and salt stress, there was no significant difference in root length between wild type and mutant. It is indicated that the HSI gene is involved in the growth process of the root, and the regulatory role of the gene needs to be further clarified.