菩人丹超微粉对STZ诱导糖尿病大鼠视网膜病变的保护作用及其对NF-κB信号通路的影响

Protective effect of Purendan Superfine Powder on retinopathy of rats with diabetes mellitus induced by streptozotocin and its influence in NF-κB signaling pathway

目的:探讨菩人丹超微粉(PRD)对糖尿病大鼠视网膜的保护作用及其对核因子κB(NF-κB)信号通路的影响,并阐明其作用机制。方法:40只Wistar大鼠随机分为正常对照组、模型组、低剂量PRD组和高剂量PRD组。给药3个月后,测定各组大鼠血糖水平,RT-PCR法检测大鼠视网膜组织中血管内皮生长因子(VEGF)和血管生成素2(Ang-2)mRNA表达水平,Western blotting法检测大鼠视网膜组织中晚期糖基化终末产物(AGEs)、晚期糖基化终末产物受体(RAGE)、NF-κB、VEGF和Ang-2蛋白表达水平。结果:与正常对照组比较,模型组大鼠血糖水平及视网膜组织中VEGF、Ang-2mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显升高(P<0.05);与模型组比较,低和高剂量PRD组大鼠血糖水平及视网膜组织中VEGF、Ang-2 mRNA表达水平和AGEs、RAGE、NF-κB、VEGF、Ang-2蛋白表达水平明显降低(P<0.05)。结论:PRD可能通过特异性阻断AGEs/RAGE/NF-κB信号通路对糖尿病大鼠视网膜起保护作用。

Objective：To investigate the protective effect of Purendan superfine powder（PRD）on the retinopathy of the diabetic rats and its influence in the nuclear factor kappa B（NF-κB）signaling pathway,and to elucidate its mechanism of action.Methods：Forty Wistar rats were randomly divided into normal control group,model group,low dose of PRD group and high dose of PRD group.After 3 months of administration,the blood sugar levels of the rats in various groups were detected.The expression levels of vascular endothelial growth factor（VEGF）and angiogenin-2（Ang-2）mRNA in retina tissue of the rats were determined by RT-PCR.The expression levels of advanced glycation end products（AGEs）,receptor for advanced glycation end products（RAGE）,NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats were determined by Western blotting method.Results：Compared with normal control group,the blood sugar level,the expression levels of VEGF,Ang-2 mRNA in retina tissue and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina of the rats in model group were significantly increased（P〈0.05）.Compared with model group,the blood sugar levels,the expression levels of VEGF and Ang-2 mRNA in retina tissue,and the expression levels of AGEs,RAGE,NF-κB,VEGF,Ang-2 proteins in retina tissue of the rats in low and high doses of PRD group were significantly decreased（P〈0.05）.Conclusion：PRD may protect the retina of diabetic rats by specifically blocking the AGEs/RAGE/NF-κB signaling pathway.