4种磷脂囊泡的细胞毒性及促胰岛素细胞内转运的研究

Cytotoxicity and Insulin Intracellular Delivery of Four Kinds of Phospholipid Vesicles

目的探讨4种磷脂囊泡对人肺腺癌细胞(A549)的细胞毒性及促细胞内胰岛素转运的影响。方法 4种胰岛素磷脂囊泡处理A549细胞,采用噻唑蓝比色法(MTT)检测磷脂囊泡细胞毒性,电化学发光法(ECLIA)检测细胞内胰岛素含量。结果 MTT实验结果显示胰岛素浓度≤0.2 U·m L-1时细胞存活率>95%,50%胰岛素磷脂囊泡浓度对细胞存活率的影响以胰岛素脂质体组最高(62.48±2.46)%,乙醇脂质体组最低(16.85±5.05)%,相对安全性顺序依次为:脂质体>脂质微泡>丙二醇脂质体>乙醇脂质体。相同浓度下4种磷脂囊泡促细胞内胰岛素转运的含量排序依次为:脂质微泡>丙二醇脂质体>乙醇脂质体>脂质体。结论以磷脂类膜材构建的脂质体、脂质微泡具备较高安全性,脂质微泡促进胰岛素细胞内转运能力最强。

Objective To study the cytotoxicity and insulin intracellular delivery of four kinds of phospholipid vesicles as liposome,ethanol liposome,propylene glycol liposome,and lipid microbubble in human lung adenocarcinoma cell line（ A549）. Methods A549 cells were treated with four kinds of insulin phospholipid vesicles. Methyl thiazolyl tetrazolium（ MTT） chromatometry method was employed to determine the cytotoxicity of phospholipid vesicles. The content of intracellular insulin was detected by electrochemical luminescence method（ ECLIA）. Results MTT experimental results showed that when the insulin concentration was less than 0.2 U·mL -1 , the cell survival rate was more than 95%. When concentration of insulin phospholipid vesicles was 50%, the highest cell survival rate was in insulin liposome group （62.48±2.46）%; the lowest cell survival rate in ethanol liposome group （16.85±5.05）%. The relative safety order was liposomes, microbubbles, propylene glycol liposomes, and ethanol liposomes. At the same concentration of phospholipid vesicles, the order of insulin intracellular contents was： lipid microbubbles〉propylene glycol liposomes〉ethanol liposomes〉liposomes.Conclusion Liposomes and lipid microbubbles constructed with phospholipid membrane are of high safety. Lipid microbubbles have the strongest ability in insulin intracellular delivery.