微喷条件下葡萄叶绿素质量比及荧光参数变化特征

Chlorophyll content and fluorescence parameters of grape under micro spray conditions

为研究极端干旱区葡萄微喷条件下叶绿素质量比及荧光动力学参数的变化特征,通过对葡萄各生育期叶绿素质量比和叶绿素荧光的测定,分析了微喷条件下不同处理的叶绿素荧光的日变化特征、叶绿素质量比变化及叶绿素质量比与荧光参数的相关性.结果显示:葡萄在坐果期、果实膨大期和果实成熟期的叶绿素质量比(Chl)及荧光参数差异具有统计学意义,Chl和F_v/F_m的变异系数均较小,分别为0.013~0.017和0.009~0.010,F_v/F_o的变异系数最大,为0.026~0.029;在叶绿素与荧光参数的关系上,F_v与_Fo,F_m,F_v/F_m,F_v/F_o和F_v/F_m与F_v/F_o呈显著正相关,相关系数为0.157~0.999.F_o/F_m与F_m,F_v,F_v/F_o,F_v/F_m均呈负相关,相关系数为-0.143^-0.992;随着喷水周期增大,F_v/F_m和F_v/F_o的实测值降低明显,表明适宜的喷水时长和周期可改善葡萄的叶绿素荧光特性,从而准确反映水分胁迫效应.

In order to study the chlorophyll content （Chl） and variation characteristics of fluorescence kinetic parameters of grape under micro spray condition in extreme drought land, the diurnal variation of chlorophyll fluorescence, Chl variation and the correlation between Chl and fluorescence parameters were analyzed by measuring the Chl and chlorophyll fluorescence in various growth stages of grape. The results show that there are significant differences in Chl and fluorescence parameters in fruit setting, expansion and maturity periods. The variation coefficients of Chl and Fv/Fm are all as small as 0. 013 - 0. 017 and 0. 009 -0. 010, respectively. The variation coefficient of Fv/Fo is in 0. 026 -0. 029 and is the largest. On the relationship between Chl and fluorescence parameters, Fv and Fo, Fm, Fv/Fm, Fv/Fo and Fv/Fm are significantly and positively correlated to Fv/Fo, with a correlation coefficient of 0. 157 -0. 999. Fo/Fm is negatively correlated to Fm, Fv, Fv/Fo and Fv/Fm, and the correlation coefficient is in the range of -0. 143 - -0. 992. With the increase of water spraying duration, the measured values of Fv/Fm and Fv/Fo decrease obviously, indicating that an appropriate water spraying duration and period can improve the chlorophyll fluorescence characteristics of grape, thus the water stress effect can be reflected exactly.