金纳米花的快速可控制备及应用

A Rapid Method for Preparing Gold Nanoflower and Its Application

采用一步合成法,以十六烷基乙二胺三乙酸(HED3A)为表面活性剂,氯金酸为金源,抗坏血酸(AA)为还原剂,在室温下高效制备形貌可控的金纳米花。改变HED3A、抗坏血酸、氯金酸的浓度和反应温度,实现对金纳米花形貌的调控。研究发现:在其他条件相同,当HED3A的浓度从0.1 mmol/L增加到50 mmol/L时,金纳米颗粒由花形变成了球形;当AA的浓度从0.06 mol/L增加到0.2 mol/L时,金纳米花由致密变得疏松;氯金酸浓度从0.5 mg/m L增加到3 mg/m L时,金纳米颗粒由球形变成花形;温度20°C升高到80°C时,金纳米颗粒由花形变成了球形;金纳米花稳定12个月之后,形貌无变化。以金纳米花为表面增强拉曼基底材料,罗丹明6G为拉曼探针,检出限可达10 nmol/L;并且细胞毒性实验表明其细胞毒性小。结果表明,使用该表面活性剂可有效调控金纳米花的形貌,制得的金纳米花在表面增强拉曼基底和生物材料等方面具有一定的应用前景。

In this paper, hexadecylethylenediaminetriacetic acid（HED3A）was used as the surfactant to prepare gold nanometer flowers for the first time. The appearance of gold nanometer flowers could be effected by the concentration of HED3A, ascorbic acid（AA） and chloroauric acid（HAuCl 4）and the reaction temperature. The results showed that under the same conditions, when the concentration of HED3A increased from 0.1 mmol/L to 50 mmol/L, the gold nanoparticles changed from flower to sphere; When the concentration of ascorbic acid increased from 0.06 mol/L to 0.2 mol/L, the gold nanoflowers from density to loose; when the concentration of HAuCl 4 increased from 0.5 mg/ml to 3 mg/ml, the gold nanoparticles changed from spherical to flower; when the temperature increased from 20 ℃ to 80℃, the gold nanoparticles changed from flower to sphere. The morphology of gold nanoparticles remained unchanged after 12 months. The surface enhanced Raman spectrum（SERS）of Rhodamine 6G（R6G）was detected to 10 nmol/L and the gold nanometer flowers were proved non-toxic. The above results show that the surfactant can effectively control the morphology of gold nanoflowers, and the obtained gold nanoflowers may be a potential Raman enhanced substrate and biomedical materials.