微流控芯片非接触电导法快速测定盐酸多塞平

Rapid detection of doxepin hydrochloride by microchip capillary electrophoresiswith contactless conductivity detection

建立了微流控芯片毛细管电泳非接触电导检测法快速测定片剂中盐酸多塞平的方法。探讨了缓冲液类型和浓度、分离电压、进样时间等因素对分离检测的影响。采用4.0 mmol·L^(-1)醋酸-5.0 mmol·L^(-1)醋酸钠(pH=4.78)为缓冲溶液,分离电压2.0 kV,在2.0 min内实现了盐酸多塞平的快速分离测定。在10.0~110.0μg·mL^(-1)范围内,峰面积与浓度呈良好的线性关系,检出限为2.0μg·mL^(-1)(S/N=3),RSD为1.0%,其片剂加标回收率为97.7%~101.6%。该方法快速简便,重现性好,适用于快速测定盐酸多塞平的含量。

A method for the rapid determination of doxepin hydrochloride by chip capillary electrophoresis with contactless conductivity detection was built in this article.Effects of the type and concentration of buffer,separation voltage and injection time on the separation and detection were investigated.Doxepin hydrochloride was separated and detected in the buffer solution of 4. 0 mmol·L^-1 HAc-5. 0 mmol·L^-1 Na Ac（ pH = 4. 87） at a separation voltage of 2. 0 kV within 2. 0 mins.The peak area showed a good linear relationship with the concentration of doxepin hydrochloride ranged from 10. 0 μg·mL^-1 to 110. 0 μg·mL^-1,the limit of detection reached 2. 0 μg·mL^-1（ S/N= 3）,the RSD was 1. 0%. The recoveries of tablets were 97. 7% - 101. 6%. The method can be used for the rapid detection of doxepin hydrochloride due to its simplicity,rapidity and reproducibility.