纳洛酮对果糖偏好大鼠自主活动和自主运动行为影响的研究

Effect of Naloxone on Locomotor Activity and Voluntary Exercise of Fructose Preference Rats

目的:研究阿片肽受体阻断剂纳洛酮对果糖偏好大鼠摄食活动、自主活动和主动运动行为的影响,探讨阿片肽系统在异常摄食和运动行为调节中的作用。方法:双瓶测试、24h液体摄入量测试评价果糖偏好动物模型状态。纳洛酮注射后采用美国Columbus代谢分析系统监测摄食饮水和自主活动,主动转轮系统监测大鼠自主运动行为参数。结果:(1)双瓶测试和24h果糖摄入量结果显示,模型组大鼠果糖摄入量高于对照组大鼠(P<0.05)。模型组大鼠24小时果糖溶液的摄入量高于饮水量,注射纳洛酮后果糖摄入量下降(P<0.05),但仍高于饮水量,同时摄食量下降(P<0.05)。(2)模型组大鼠果糖喂养期间24小时自主活动高于饮水状态(P<0.05),注射纳洛酮后自主活动下降(P<0.05),并且与饮水状态下自主活动水平无差异。(3)以模型组大鼠饮水状态下24小时自主运动量为参照,果糖喂养期间模型大鼠自主运动量显著下降,注射纳洛酮后自主运动量增多(P<0.05)。结论:阿片肽系统参与果糖偏好大鼠果糖的奖赏效应下自主活动增多,自主运动行为抑制的中枢调节过程。

Purpose： This study measures the change of feeding behavior, locomotor activity and voluntary- exercise of fructose preference rats, and analyzes the central effect of endogenous opioid peptides （EOP） system with EOP blocker naloxone. Methods ： The fructose preference rat model was established induced by LiC1 under ad libitum feeding condition. Two - bottle test and 24 - h fructose intake were conducted to confirm the reliability of the modeh Locomotors activity of rats was measured in the Columbus metabolic analysis system cages （CLAMS; Columbus, OH, USA）, and voluntary exercise was monitored by wheel running system. Naloxone （3 mg/kg, i. p. ; Sigma, St. Louis, MO, USA） was injected before test. Results： Two- bottle test and 24 - h fructose solution intake showed the BED model rats had higher fructose preference and larger amount fructose solution intake than that of normal rats （ P 〈 0.05 ）, suggested that the BED model established successfully. BED model rat displayed more fructose solution intake than that of water intake for 24 - h period, which decreased fructose intakewith naloxone （ P 〈 0.05 ）, accompanied with food intake reducing （ P 〈 0.05 ）. Locomotors activity- of BED model rats was higher in the fructose solution available than that in the water drinking state （ P 〈 0.05）. Naloxone decreased the locomotors activity- of BED model rats （ P 〈 0.05 ） , which was no difference with water drinking state. Voluntary exercise of BED model rats showed completely opposite change compared with locomotors activity. Conclusion： EOP system involves in the central regulation mechanism of fructose addiction rats with autonomic activity increased and voluntary exercise behavior inhibition.