常压室温等离子体诱变及高效筛选异麦芽酮糖高产菌株

Screening mutation of high isomaltulose producing strain using atmospheric and room temperature plasmas

以一株实验室筛选的Klebsiellasp.LX3为出发菌株,采用常压室温等离子体(ARTP)注入技术对菌株进行诱变,并通过测定发酵液中蔗糖异构酶活性、异麦芽酮糖含量以及检测菌体絮凝效果,获得一株异麦芽酮糖高产且黏度较低的菌株,命名为LX3-1。实验结果表明,相比野生菌,突变株蔗糖异构酶酶活提高了20.42%(P<0.05),异麦芽酮糖产量提高了41.87%,絮凝后菌体去除率较野生菌提高了6.9%。突变株经6次传代培养后,发酵液中蔗糖异构酶酶活和异麦芽酮糖产量稳定。研究结果表明,新型的ARTP诱变技术对提高蔗糖异构酶活力、降低菌体黏度的作用比较明显。

A mutant strain LX3-1 was obtained using Klebsiella sp.LX3 as a start strain by atmospheric and room temperature plasma（ARTP）.The results of sucrose isomerase activity,isomaltulose yield and flocculation effect confirmed that LX3-1 is a high isomaltulose-producing strain with lower viscosity.Compared with the wild type,the sucrose isomerase activity,isomaltulose yield,bacteria removal rate after the flocculation of LX3-1 increased by 20.42%,41.87%and 6.9%,respectively.After six passage cultivation times,LX3-1 maintained stable sucrose isomerase activity and isomaltulose yield.The results showed that ARTP had an obvious impact on improving enzyme activity and lowering bacteria viscosity,which could accelerate the commercial process of isomaltulose.