光信号对Fmr1 KO小鼠睡眠-觉醒的调控作用

Effects of light signal on sleep-wake in Fmr1 KO mice

目的探讨光授时信号对脆性X智力低下基因型(Fmr1 KO)小鼠睡眠-觉醒的影响。方法采用小鼠脑立体定位技术埋置脑电和肌电电极,记录和分析在不同光照条件下Fmr1 KO小鼠和野生型(WT)小鼠的睡眠-觉醒节律的变化。结果Fmr1 KO小鼠和WT小鼠在正常光照(12 h∶12 h明暗,即8:00 am开灯,8:00 pm关灯)条件下的觉醒和睡眠均呈现一致的昼夜节律现象,且二者之间差异无统计学意义;与正常光照的同样时间段相比较,9:00 am^11:00 am关灯期间,Fmr1 KO小鼠和WT小鼠的觉醒量均显著增加(P<0.05),且在9:00 am^10:00 am变化极显著(P<0.01),但Fmr1 KO小鼠觉醒的增加量明显低于WT小鼠(P<0.05);与正常光照的同样时间段相比较,9:00 am^11:00 am关灯期间,Fmr1 KO小鼠和WT小鼠的慢波睡眠(SWS)-觉醒(W)、W-SWS和SWS-快波睡眠(FWS)时相转换量都显著增加(P<0.05),其中在9:00 am^10:00 am极显著增加(P<0.01)。结论相比于WT小鼠,Fmr1 KO小鼠的睡眠-觉醒受光信号改变的影响有所减弱。

Objective To explore the effect of light signal on sleep-wake in Fmr1 KO mice. Methods The changes of sleep-wake rhythm in different light conditions were recorded and analyzed through EEG and EMG by using the stereotopic localization technique in Fmr1 KO and WT mice,respectively. Results Under the normal light condition（ 12 h ∶ 12 h light-dark cycle,turning on the light at 8： 00 am,and turning off the light at 8： 00 pm）,wakefulness and sleep were shown in consistent phenomenon of circadian rhythm,and no statistically significant difference was observed between Fmr1 KO and WT mice; Compared with that in the same period under normal light condition,the wakefulness of Fmr1 KO and WT mice were both increased within the two hours from 9： 00 am to 11： 00 am（ P 〈 0. 05）,and the change within 9： 00 am ~ 10： 00 am was remarkably significant difference（ P 〈0. 01）. However,compared with that of WT mice,the increased wakefulness of the Fmr1 KO mice was lower（ P〈 0. 05）; Compared with that in the same period under the normal condition,slow wave sleep（ SWS）-wakefulness（ W）,W-SWS,and SWS-fast wave sleep（ FWS） phase transformations were increased significantly in both Fmr1 KO mice and WT mice under the condition of turning off the light for 2 hours from 9： 00 am to 11： 00 am,and the change within 9： 00 am ~ 10： 00 am was remarkably significant difference（ P 〈 0. 01）. Conclusion The sleepwake of Fmr1 KO mice is not susceptible to light signal compared with WT mice.