p38信号通路在三叉神经痛ION-CCI模型大鼠中的研究

Role of p38 signaling pathway in the trigeminal neuralgia of ION-CCI model in rats

目的检测三叉神经痛(TN)模型大鼠三叉神经节(TG)内p-p38蛋白表达量的变化。方法采用眶下神经缩窄术(ION-CCI)制备大鼠三叉神经痛模型,将雄性SD大鼠随机分为两组,即对照组(sham组)和眶下神经缩窄术组(ION-CCI组)。Frey毛刷测定大鼠触须垫机械痛阈,Western blot检测p-p38的表达量。结果 ION-CCI组大鼠机械痛阈明显低于sham组(n=5,P<0.01)。ION-CCI组大鼠TG内p-p38的蛋白表达量均明显高于建模前正常大鼠(n=3,P<0.05)。结论 p38信号通路可能与TN的发生发展密切相关。

Objective To detect the expression of p-p38 proteins in intrigeminal ganglions（ TG） of trigeminal neuralgia（ TN） model in rats. Methods The TN model of rat was established by chronic constriction injury of the infraorbital never（ ION-CCI）. Male SD rats were randomly diviede into 2 groups： control group（ sham group） and ION-CCI group. The mechanical withdrawal thresholds of vibrissa pad in two groups of rats were measured by von Frey hairs. The proteins of p-p38 in TG were detected by Western blot test. Results The mechanical thresholds of ION-CCI group were obviously lower than that in sham group（ n = 5,P 〈 0. 01）. The p-p38 proteins in TG of IONCCI group was over-expressed obviously after operation,which was significant different compared with that before operation（ n = 3,P 〈 0. 05）. Conclusion p38 signaling pathway may be closely related to the development of TN.