新型靶向药物对A549细胞体外抑制作用及其能谱CT成像初步研究

The preliminary study on effect of targeting drugs on human non-small cell lung cancer A549 cells in vitro and quantification of iodine with Gemstone Spectral Imaging

目的探讨新型靶向药物对人非小细胞肺癌A549细胞的体外抑制作用及其能谱CT成像能力。方法合成具有靶向A549细胞毒性的靶向药物,使用2.5、5、10、20、40μmol/L靶向药物及吉非替尼分别体外培养A549细胞,应用四甲基偶氮唑蓝(MTT)比色法检测细胞抑制率。对0、1.87、3.75、7.5、15、30 mmol/L靶向药物及吉非替尼进行能谱CT GSI模式扫描,分析两种药物细胞增殖抑制率与CT值差别及靶向药物碘浓度测量值与真实值之间关系。结果靶向药物及吉非替尼可随时间及剂量依赖性地抑制A549细胞增殖,浓度超过10μmol/L时,靶向药物抑制率高于吉非替尼(P<0.05);能谱CT GSI模式扫描,靶向药物CT值及碘浓度值明显高于吉非替尼(P<0.05);靶向药物碘浓度测量值与真实值之间具有明显相关性(r=0.998)。结论新型靶向药物在很好的抑制A549细胞的同时,又具有良好的CT成像性能;利用能谱CT基物质成像能够准确反映不同浓度溶液的碘含量。

Objective To explore the effects of targeting drug on human non-small cell lung cancer A549 cells in vitro and quantification of iodine with gemstone spectral imaging（ GSI）. Methods In the first part,synthesis of small iodine-containing drugs with cytotoxicity targeting A549 cells. A549 cells were treated in vitro with targeting drugs and gefitinib at a concentration gradient of 2. 5,5,10,20,40 μmol/L. Their proliferation effects were analyzed by the thiazolyl blue（ MTT） assay. In the second part,targeting drugs and gefitinib at a concentration gradient of 0,1. 87,3. 75,7. 5,15,30 mmol/L underwent GE HD750 CT with GSI mode. Analyzing the inhibition rate of two kinds of drug cell proliferation,comparing the relation and discrepancy between measured iodine concentrations and real iodine concentrations. Results In the first part,Targeting drugs and gefitinib induced a dose-and timeindependent growth inhibition by MTT. When the concentration was more than 10 μmol/L,the inhibitory rate of targeting drugs was higher than that of gefitinib（ P 〈 0. 05）. In the second part,in a certain range of concentration,the CT value and iodine concentration of targeting drugs were significantly higher than that of gefitinib（ P 〈 0. 05）.The measured concentrations of targeting drugs were obviously correlated to the real concentrations（ r = 0. 998）without significant difference between these two values（ P 〉 0. 05）. Conclusion The targeting drug has good inhibitory effect on A549 cells in vitro. The high CT value of targeting drugs is good for CT shows. GSI of spectral CT is a reliable method to quantify iodine of the targeting drugs.