血站型滤器法制备去除白细胞血液质量控制探讨

Study on Blood Quality Control of Blood Station Type Filter Preparation in Removing the Leukocyte

目的通过控制滤除白细胞操作过程的关键控制点,了解其对滤白血液质量的影响。方法随机抽取2016年9—12月份献血屋采集的400 mL全血80袋,按照在滤除白细胞过程中是否对其关键点进行有效控制分为实验组和对照组,比较两组过滤后的血液损失量和过滤效果。结果实验组和对照组过滤后的血液损失量分别为(43.47±1.45)、(44.83±3.45)g,差异有统计学意义(t=-2.296,P<0.05);两组制备后的去白细胞悬浮红细胞的容量分别为(295.68±18.61)、(293.30±17.13)(t=0.644);血红蛋白含量分别为(55.20±7.18)、(54.55±5.24)(t=0.589);白细胞残留量分别为(1.65±0.87)、(1.64±0.77)(t=0.027);储存期末溶血率分别为(0.082±0.045)%,(0.087±0.050)%(t=-0.451),差异无统计学意义(P>0.05),结果均符合《全血及成分血质量要求》(GB18469-2012)。结论注重滤白关键操作点,可以降低血液损失量,但对过滤血液的质量无影响。

Objective To study the effect on post-filtration blood quality by controlling the key control points in the operation process of leukocyte removal. Methods 80 bags of 400 mL whole blood from blood donation room from September to December 2016 were randomly selected and divided into experimental group and control group, according to whether the key points were effectively controlled in the filtering process. The blood loss and filtration effect were compared between the two groups. Results The loss of blood in experimental group and control group after filtration was（43.47 ±1.45） and（44.83±3.45）g respectively, and there was a significant difference in the two groups（t=-2.296, P0.05）. The hemoglobin contents of leukoreduced suspening erythrocytes after preparation were（295.68±18.61）,（293.30±17.13）（t=0.644）; the contents of hemoglobin were respectively（55.20±7.18）,（54.55±5.24）（t=0.589）, the residual leucocyte contents were（1.65±0.87） and（1.64 ±0.77）（t =0.027）; the hemolytic ratios at the end of storage were（0.082 ±0.045）%,（0.087 ±0.050）%（t =-0.451）. and there was no significant difference between the two groups（P〉0.05）,And the results met the requirements of Quality requirements for Whole Blood and Blood Components（GB18469-2012）. Conclusion Paying attention to the leukocyte-filtered key operation points can reduce the loss of blood, but there is no effect on the quality of filtered blood.