化瘀解毒方对Lewis肺癌小鼠免疫功能及E-cadherin、Vimentin蛋白表达的影响

Effect of Huayu Jiedu Decoction on Immune Function and Expression of E-cadherin and Vimentin in Lewis Lung Cancer Mice

目的:观察化瘀解毒方对Lewis肺癌小鼠肿瘤生长、免疫功能及E-cadherin、Vimentin蛋白表达的影响。方法:50只雄性SPF级C57BL/6小鼠随机分组为模型组、环磷酰胺组、环磷酰胺联合化瘀解毒方组、化瘀解毒方高剂量组、化瘀解毒方低剂量组,建立Lewis肺癌移植瘤模型,给药14 d后取肿瘤组织并称质量,计算各组抑瘤率,采用酶联免疫吸附法检测血清中白细胞介素-2(interleukin-2,IL-2)、白细胞介素-10(interleukin-10,IL-10)、γ-干扰素(interferon-γ,IFN-γ)的含量,Western blot法观察肿瘤组织中E-cadherin和Vimentin的表达情况。结果:与模型组比较,环磷酰胺联合化瘀解毒方组、化瘀解毒方高剂量组能抑制肿瘤生长,且血清中IL-2和IFN-γ水平升高,IL-10水平降低,肿瘤组织中E-cadherin表达增强,差异均有统计学意义(P<0.05或P<0.01)。结论:化瘀解毒方对Lewis肺癌小鼠肿瘤的生长具有一定的抑制作用,并能调节免疫细胞因子和E-cadherin的表达。

Objective： To study the effects of Huayu Jiedu（ HYJD） decoction on the growth of tumor,immune function and the expression of E-cadherin,Vimentin in mice with Lewis lung cancer. Methods： The Lewis lung cancer model was established in male C57 BL/6 mice. Fifty C57 BL/6 mice were randomly divided into model group,cytoxan group,HYJD combined with cytoxan group,and high,low dose HYJD groups. The drugs were administred orally for 14 days after modeling. Then the mice were sacrificed and the tumor inhibition rate of HYJD was calculated according to the final tumor weight. The concentration of serum interleukin-2（ IL-2）,interleukin-10（ IL-10）,γ-interferon（ IFN-γ） were measured by enzyme-linked immuno sorbent assay（ ELISA）. The expression of E-adherin and Vimentin were detected by Western blot. Results： Compared with the model group,the tumor inhibition rate and the levels of IL-2 and IFN-γ in HYJD combined with cytoxan group,high and low dose HYJD groups were significantly increased. The contents of IL-10 increased and the expression of E-cadherin enhanced（ P 〈 0. 05,P 〈 0. 01）. Conclusion： HYJD showed inhibitory effect on the growth of tumor of mice with Lewis lung cancer it could make an effect on the level of cytokine and increase the expression of E-cadherin.