参苓白术散对高脂血症小鼠甘油三脂水平的影响研究

Effect of Shenlingbaizhu San on High Fat Fed Mice about Triacyglyceride

目的:探讨参苓白术散对高脂血症小鼠甘油三酯以及基础指标(体重、血糖)等的影响。方法:将8～10周龄C57BL/6小鼠随机分为对照组(普通饲料+生理盐水灌胃)、模型组(高脂饲料+生理盐水灌胃)、参苓白术散组(高脂饲料+参苓白术散灌胃,灌胃剂量10g/kg)。高脂饲养小鼠12周,每周监测体重、血糖指标,于实验结束时收取血浆,测定血液中葡萄糖、甘油三脂、胰岛素水平。在收取组织前做胰岛素耐量(ITT)、葡萄糖耐量(GTT)实验以评估胰岛功能及胰岛素敏感性。结果:与对照组小鼠比较,模型组小鼠甘油三脂水平均显著增高(P<0.05),参苓白术散组能明显降低高脂造成的高甘油三脂血症,且差异有统计学意义(P<0.05)。模型组小鼠血糖及体重较对照组明显升高(P<0.05),葡萄糖耐量呈现受损趋势,这一改变并未因参苓白术散应用而改变。参苓白术散组小鼠血液中胰岛素水平高于模型组小鼠。结论:参苓白术散可改善高脂喂养小鼠的高甘油三脂血症,血糖及体重无改善,可能与药物剂量存在一定关系。

Objective： To investigate the effect of Shenlingbaizhusan on mice with triacyglyceride and based indicators （weight, blood sugar） and so on. Methods： 8-10 weeks old C57BL/6 mice were randomly divided into control group （normal diet ＋ saline）, model group （high fat diet ＋ saline gavage）, shenlingbaizhusan group （high fat diet ＋shenlingbaizhusan ,intragastric gavage dose 10g/kg）. Mice with high fat diet for 12 weeks, and their body weight and blood sugar levels were monitored every week. Plasma was collected at the end of the experiment, and blood glucose, triacyglyceride and insulin levels were measured. Insulin tolerance （ITT） and glucose tolerance （GTT） tests were performed to assess islet function and insulin sensitivity before the tissue collection. Results：Compared with control group mice, model group mice triacyglyceride levels were significantly increased （P 〈0.05）, shen- lingbaizhusan group can obviously reduce thetriacyglyceride level, and the difference was statistically significant （ P〈0. 05）. The model group the blood glucose and body weight were significantly higher than those in control group （P〈0.05）, Glucose tolerance showed an impaired trend,This change is not changed because of sbenlingbaizhusan application. Conclusion ： Sbenlingbaizhusan can improve the Hypertriglyceridemia, blood glucose and body weight did not improve may have some relationship with the dose of the drug.