摘要
目的:建立脑脉通方剂中川芎有效成分的提取工艺和含量测定方法,以有效控制其质量。方法:分别采用氯化铁-铁氰化钾比色法、酸性染料比色法和高效液相色谱法测定川芎中总酚酸、总生物碱、阿魏酸的含量,并对3批川芎中的有效成分进行定量分析。结果:川芎有效成分中可测成分含量占总含量的50%以上,符合有效成分的要求,其中总酚酸的含量为51.82%~53.46%,总生物碱的含量为7.50%~7.67%,阿魏酸含量为2.23%~2.29%。结论:所建立的方法操作简便,精密度和重复性好,可作为脑脉通中川芎有效成分的质量控制方法。
Objective: To accurate evaluate extraction process of the effective part of chuanxiong of naomaitong formula, establish an effective method to control its quality. Methods:with ferulic acid and hydrochloric acid ligustrazine as evaluation index, to estah lish content determination method of total phenolic acid, total alkaloids, ferulic acid of the effective part of chuanxiong with ferric chloride and potassium ferricyanide colorimetric method, the acid dye colorimetric method and high performance liquid chromatogra phy (HPLC) methods, meanwhile,quantitative analysis of 3 batches of the effective part of chuanxiong. Results:The effective part of chuanxiong measured component content more than 50% of the total content, accord with the requirement of effective part, of whichthe content of total phenolic, alkaloid and ferulic acid were 51.82%-53.46%,7.50%-7.67% and 2.23%-2.29%. Conclusion:The method is simple, precision and the repeatability is good, and they can be used for quality control of the effective part of chuanxiong of naomaitong formula.
作者
李克宁
李恒
郑惠婷
王淑美
陈磊
Li Kening;Li Heng;Zheng Huiting;Wang Shumei;Chen Lei(School of Traditional Chinese,Medicine,Guangdong Pharmaceutical University Guangzhou 510006 China;Key Laboratory of Traditional Chinese Medicine Oigitalization Quality Evaluation under State Administration of Traditional Chinese Medicine,Guangzhou 5100,06,China;Research Center for Traditional Chinese Medicine Quality Engineering Technology of Guangdong Universities,Guangzhou 510006,China)
出处
《亚太传统医药》
2018年第7期29-34,共6页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金资助项目(81073024
81274060)
关键词
脑脉通
川芎
有效成分
质量控制
Naomaitong
Chuanxiong
Effect Parts
Quality Control